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The Synergistic Apoptotic Interaction of Indole-3-Carbinol and Genistein with TRAIL on Endometrial Cancer Cells

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Author(s)
Bidur ParajuliSo-Jin ShinSang-Hoon KwonSoon-Do ChaHyun-Gyo LeeInsoo BaeChi-Heum Cho
Keimyung Author(s)
Shin, So JinKwon, Sang HoonCha, Soon DoCho, Chi Heum
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Institute for Cancer Research (암연구소)
Journal Title
Journal of Korean Medical Science
Issued Date
2013
Volume
28
Issue
4
Keyword
ApoptosisIndole-3-CarbinolGenisteinTRAILEndometrial Neoplasms
Abstract
Induction of apoptosis in target cells is a key mechanism by which chemotherapy promotes cell killing. The purpose of this study was to determine whether Indole-3-Carbinol (I3C) and Genistein in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induce apoptosis in endometrial cancer cell (Ishikawa) and to assess apoptotic mechanism. The MTT assay and flow cytometry were performed to determine cell viability and cell cycle. The induction of apoptosis was measured by caspase-3 activity test, DNA fragmentation assay, annexin V binding assay and western blot analysis. There was no effect in cell growth inhibition and cell cycle progression alone or in two-combination. However, the treatment of I3C and Genistein followed by TRAIL showed significant cell death and marked increase in sub-G1 arrest. Three-combination treatment revealed elevated expression of DR4, DR5 and cleaved forms of caspase-3, caspase-8, PARP. The Flip was found down regulated. Moreover, increase in caspase-3 activity and DNA fragmentation indicated the induction of apoptosis. The results indicate that I3C and Genistein with TRAIL synergistically induced apoptosis via death receptor dependent pathway. Our findings might provide a new insight into the development of novel combination therapies against endometrial cancer.
Keimyung Author(s)(Kor)
신소진
권상훈
차순도
조치흠
Publisher
School of Medicine
Citation
Bidur Parajuli et al. (2013). The Synergistic Apoptotic Interaction of Indole-3-Carbinol and Genistein with TRAIL on Endometrial Cancer Cells. Journal of Korean Medical Science, 28(4), 527–533. doi: 10.3346/jkms.2013.28.4.527
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2013.28.4.527
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34131
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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