Lysophosphatidylcholine as a death effector in the lipoapoptosis of hepatocytes
- Author(s)
- Myoung Sook Han; Sun Young Park; Koei Shinzawa; Sunshin Kim; Kun Wook Chung; Ji-Hyun Lee; Choon Hyuck Kwon; Kwang-Woong Lee; Joon-Hyoek Lee; Cheol Keun Park; Woo Jin Chung; Jae Seok Hwang; Ji-Jing Yan; Dong-Keun Song; Yoshihide Tsujimoto; Myung-Shik Lee
- Keimyung Author(s)
- Chung, Woo Jin; Hwang, Jae Seok
- Department
- Dept. of Internal Medicine (내과학)
- Journal Title
- Journal of Lipid Research
- Issued Date
- 2008
- Volume
- 49
- Issue
- 1
- Keyword
- fatty acids; phospholipase A2; steatohepatitis; triglyceride; ceramide
- Abstract
- The pathogenesis of nonalcoholic steatohepatitis (NASH) is unclear, despite epidemiological data implicating FFAs. We studied the pathogenesis of NASH using lipoapoptosis models. Palmitic acid (PA) induced classical apoptosis of hepatocytes. PA-induced lipoapoptosis was inhibited by acyl-CoA synthetase inhibitor but not by ceramide synthesis inhibitors, suggesting that conversion products other than ceramide are involved. Phospholipase A2 (PLA2) inhibitors blocked PA-induced hepatocyte death, suggesting an important role for PLA2 and its product lysophosphatidylcholine (LPC). Small interfering RNA for Ca2+-independent phospholipase A2 (iPLA2) inhibited the lipoapoptosis of hepatocytes. PA increased LPC content, which was reversed by iPLA2 inhibitors. Pertussis toxin or dominant-negative Gαi mutant inhibited hepatocyte death by PA or LPC acting through G-protein-coupled receptor (GPCR)/Gαi. PA decreased cardiolipin content and induced mitochondrial potential loss and cytochrome c translocation. Oleic acid inhibited PA-induced hepatocyte death by diverting PA to triglyceride and decreasing LPC content, suggesting that FFAs lead to steatosis or lipoapoptosis according to the abundance of saturated/unsaturated FFAs. LPC administration induced hepatitis in vivo. LPC content was increased in the liver specimens from NASH patients. These results demonstrate that LPC is a death effector in the lipoapoptosis of hepatocytes and suggest potential therapeutic values of PLA2 inhibitors or GPCR/Gαi inhibitors in NASH.
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