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Calcium Mobilization by Activation of M3/M5 Muscarinic Receptors in the Human Retinoblastoma

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Author(s)
Dae-Ran KimSang Hoon RahJoon Hyung SohnByung-Il YehChang Mann KoJeong Sook ParkMin-Jeong KimJoong Woo LeeIn Deok Kong
Keimyung Author(s)
Kim, Na HyunPark, Jeong Sook
Department
Dept. of Nursing (간호학)
Research Institute for Nursing Science (간호과학연구소)
Journal Title
Journal of Pharmacological Sciences
Issued Date
2007
Volume
105
Issue
2
Keyword
muscarinic receptorcalciumretinoblastoma
Abstract
Activation of muscarinic acetylcholine receptors (mAChR) is one of the most important signal transduction pathways in the human body. In this study, we investigated the role of mAChR activation in relation to its subtypes in human retinoblastoma cell-lines (WERI-Rb-1) using Ca2+ measurement, real-time PCR, and Western Blot techniques. Acetylcholine (ACh) produced prominent [Ca2+]i transients in a repeated manner in WERI-Rb-1 cells. The maximal amplitude of the [Ca2+]i transient was almost completely suppressed by 97.3 ± 0.8% after atropine (1 μM) pretreatment. Similar suppressions were noted after pretreatments with thapsigargin (1 μM), an ER Ca2+-ATPase (SERCA) inhibitor, whereas the ACh-induced [Ca2+]i transient was not affected even in the absence of extracellular calcium. U-73122 (1 μM), a PLC inhibitor, and xestospongin C (2 μM), an IP3-receptor antagonist, elicited 11.5 ± 2.9% and 17.8 ± 1.9% suppressions, respectively. The 50% inhibitory concentration of (IC50) values for blockade of a 100 μM ACh response by pirenzepine and 4-DAMP were 315.8 and 9.1 nM, respectively. Moreover, both M3 and M5 mAChRs were prominent in quantitative real-time-PCR. Taken together, the M3/M5 subtypes appear to be the major contributor, leading to intracellular calcium mobilization from the internal store via an IP3-dependent pathway in the undifferentiated retinoblastoma cells.
Keimyung Author(s)(Kor)
김나현
박정숙
Publisher
College of Nursing
Citation
Dae-Ran Kim et al. (2007). Calcium Mobilization by Activation of M3/M5 Muscarinic Receptors in the Human Retinoblastoma. Journal of Pharmacological Sciences, 105(2), 184–192. doi: 10.1254/jphs.FP0070877
Type
Article
ISSN
1347-8613
DOI
10.1254/jphs.FP0070877
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34231
Appears in Collections:
2. College of Nursing (간호대학) > Dept. of Nursing (간호학)
3. Research Institutues (연구소) > Research Institute for Nursing Science (간호과학연구소)
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