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Remifentanil postconditioning has cross talk with adenosine receptors in the ischemic-reperfused rat heart

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Author(s)
Jeon-Yu HaYong-Cheol LeeSang-Jin ParkYoung-Ho JangJune-Hong Kim
Keimyung Author(s)
Lee, Yong Cheol
Department
Dept. of Anesthesiology & Pain Medicine (마취통증의학)
Journal Title
Journal of Surgical Research
Issued Date
2015
Volume
195
Issue
1
Keyword
Adenosine receptorCross talkOpioid receptorPostconditioningRemifentanil
Abstract
Background:

Although there is a possibility of cross talk between opioid and adenosine signaling pathways in the ischemic-reperfused myocardium, it is not clear that an ultra-short–acting opioid receptor agonist remifentanil-induced postconditioning (RPostC) has cross talk with adenosine receptor (ADR). The purpose of this study was to determine whether there is cross talk with ADR in RPostC.


Materials and methods:

Isolated rat hearts were subjected to 30 min of regional ischemia and 2 h of reperfusion. RPostC was induced by 100 ng/mL of remifentanil perfusion, 5 min before reperfusion, followed by 5 min of reperfusion. The nonspecific opioid receptor antagonist naloxone (NAL) and the nonspecific ADR antagonist 8-(p-sulfophenyl) theophylline hydrate (8-SPT) were perfused for a 20-min period, 10 min before RPostC to the end of RPostC. Western blot analysis was performed to detect phospho-ERK1/2 in cultured cardiomyocytes.


Results:

In cultured cardiomyocytes, remifentanil incubation significantly increased the phosphorylation of ERK1/2 and this effect was blocked by both NAL and 8-SPT (P < 0.01 and P < 0.05, respectively). RPostC significantly reduced infarct size over ischemic area at risk from 34.1 ± 10.5% to 16.6 ± 7.5% (P < 0.05 versus control). The infarct-limitation effect of RPostC was reversed by both NAL (33.8 ± 13.0%, P < 0.05) and 8-SPT (35.7 ± 14.5%, P < 0.01).


Conclusions:

This study strongly implies that the intracellular signaling pathways of cardioprotection by RPostC has cross talk with ADR in the ischemic-reperfused myocardium.
Keimyung Author(s)(Kor)
이용철
Publisher
School of Medicine
Citation
Jeon-Yu Ha et al. (2015). Remifentanil postconditioning has cross talk with adenosine receptors in the ischemic-reperfused rat heart. Journal of Surgical Research, 195(1), 37–43. doi: 10.1016/j.jss.2015.01.010
Type
Article
ISSN
0022-4804
Source
https://www.sciencedirect.com/science/article/pii/S0022480415000505?via%3Dihub
DOI
10.1016/j.jss.2015.01.010
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34262
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anesthesiology & Pain Medicine (마취통증의학)
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