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Induction of Growth Inhibition and Apoptosis in Human Uterine Leiomyoma Cells by Isoliquiritigenin

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Author(s)
Dong-chul KimSabarish RamachandranSeung-hee BaekSang-Hoon KwonKun-Young KwonSoon-Do ChaInsoo BaeChi-Heum Cho
Keimyung Author(s)
Kwon, Sang HoonCha, Soon DoCho, Chi HeumKwon, Kun Young
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Dept. of Pathology (병리학)
Journal Title
Reproductive Sciences
Issued Date
2008
Volume
15
Issue
6
Keyword
Isoliquiritigeninhuman uterine leiomyomaapoptosis
Abstract
Isoliquiritigenin(ISL), a calchone flavonoid, has cancer-preventing properties and is often used in Chinese medicine. In the present study, the authors use ISL to determine its effect on cell proliferation and cell cycle progression in primary cultured human uterine leiomyoma cells. Cell viability and cell proliferation assays were conducted. Flow cytometry, annexin V apoptosis assay, and DNA fragmentation assay were performed to determine the effect of ISL on cell cycle and apoptosis. The expression of cell cycle regulatory—related proteins was evaluated by Western blot. The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by ISL treatment in a dose-dependent manner. Flow cytometry results showed that ISL induced subG1 and G2/M arrest. DNA fragmentation assay and annexin V apoptosis assays revealed apoptosis induction. ISL-induced growth inhibition in uterine leiomyoma cells was associated with increased p21Cip1/ Waf1 expression in a p53-dependent manner. Activation of caspase-3 and downregulation of Bcl-2, cdk 2/4, and E2F, with a concomitant increase in dephosphorylation of Rb and poly—ADP-ribose polymerase cleavage, were observed. This study demonstrates that ISL inhibits cell proliferation by initiating apoptosis in human uterine leiomyoma cells coupled with increased cell cycle arrest. These results indicate that ISL could prove to be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
Keimyung Author(s)(Kor)
권상훈
차순도
조치흠
권건영
Publisher
School of Medicine
Citation
Dong-chul Kim et al. (2008). Induction of Growth Inhibition and Apoptosis in Human Uterine Leiomyoma Cells by Isoliquiritigenin. Reproductive Sciences, 15(6), 552~558-552~558. doi: 10.1177/1933719107312681
Type
Article
ISSN
1933-7191
Source
http://journals.sagepub.com/doi/abs/10.1177/1933719107312681?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&
DOI
10.1177/1933719107312681
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34335
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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