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Loss of Cyclin G1 Expression in Human Uterine Leiomyoma Cells Induces Apoptosis

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Author(s)
Sang-Hoon KwonJoon-Cheol ParkSabarish RamachandranSoon-Do ChaKun-Young KwonJong-Ku ParkJong-Wook ParkInsoo BaeChi-Heum Cho
Keimyung Author(s)
Kwon, Sang HoonPark, Joon CheolCha, Soon DoCho, Chi HeumKwon, Kun YoungPark, Jong WookPark, Jong Gu
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Dept. of Pathology (병리학)
Dept. of Immunology (면역학)
Dept. of Molecular Medicine (분자의학)
Institute for Medical Science (의과학연구소)
Journal Title
Reproductive Sciences
Issued Date
2008
Volume
15
Issue
4
Keyword
Uterine leiomyomacyclin G1cyclin G1 ribbon–type antisense oligonucleotidesp53apoptosis
Abstract
Observations from the authors’ laboratory suggest a physiological role for increased cyclin G1 protein levels in human uterine leiomyoma. The hypothesis of the present study is that the strategic modulation of cyclin G1 by antisense technology will inhibit the survival of in vitro–grown uterine leiomyoma cells. Cultured uterine leiomyoma cells were transfected with cyclin G1 ribbon-type antisense oligonucleotide (cyclin G1 RiAS) to effectively reduce cyclin G1 expression. Cell viability, in situ terminal deoxyuridine nick end-labeling (TUNEL) assay, flow cytometry, DNA fragmentation, and expression of cell cycle regulatory–related proteins were evaluated by Western blot. Antisense oligonucleotides compromised uterine leiomyoma cell viability and inducted apoptosis in a caspase-independent mechanism. In situ TUNEL and DNA fragmentation revealed apoptosis induction, and fluorescent-activated cell sorting analysis showed increased sub-G1-phase cells. Furthermore, abrogation of cyclin G1 enhanced p53 accumulation, phosphorylation of p53 at Ser-15 residue, and increased expression of cyclin-dependent kinase inhibitors p21 and p27. These data imply that cyclin G1 expression is associated with growth promotion and the potential utility and novelty of using ribbon-type antisense oligonucleotides as a gene therapy strategy to treat human uterine leiomyoma.
Keimyung Author(s)(Kor)
권상훈
박준철
차순도
조치흠
권건영
박종욱
박종구
Publisher
School of Medicine
Citation
Sang-Hoon Kwon et al. (2008). Loss of Cyclin G1 Expression in Human Uterine Leiomyoma Cells Induces Apoptosis. Reproductive Sciences, 15(4), 400–410. doi: 10.1177/1933719107314063
Type
Article
ISSN
1933-7191
Source
http://journals.sagepub.com/doi/abs/10.1177/1933719107314063?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
DOI
10.1177/1933719107314063
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34336
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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