계명대학교 의학도서관 Repository

JNK activation is responsible for mucus overproduction in smoke inhalation injury

Metadata Downloads
Author(s)
Won-Il ChoiOlga SyrkinaKun Young KwonDeborah A QuinnCharles A Hales
Keimyung Author(s)
Choi, Won IlKwon, Kun Young
Department
Dept. of Internal Medicine (내과학)
Dept. of Pathology (병리학)
Journal Title
Respiratory Research
Issued Date
2010
Volume
11
Issue
1
Abstract
Background: Increased mucus secretion is one of the important characteristics of the response to smoke inhalation injuries. We hypothesized that gel-forming mucins may contribute to the increased mucus production in a smoke inhalation injury. We investigated the role of c-Jun N-terminal kinase (JNK) in modulating smoke-induced mucus secretion.
Methods: We intubated mice and exposed them to smoke from burning cotton for 15 min. Their lungs were then isolated 4 and 24 h after inhalation injury. Three groups of mice were subjected to the smoke inhalation injury: (1) wild-type (WT) mice, (2) mice lacking JNK1 (JNK1-/- mice), and (3) WT mice administered a JNK inhibitor. The JNK inhibitor (SP-600125) was injected into the mice 1 h after injury.
Results: Smoke exposure caused an increase in the production of mucus in the airway epithelium of the mice along with an increase in MUC5AC gene and protein expression, while the expression of MUC5B was not increased compared with control. We found increased MUC5AC protein expression in the airway epithelium of the WT mice groups both 4 and 24 h after smoke inhalation injury. However, overproduction of mucus and increased MUC5AC protein expression induced by smoke inhalation was suppressed in the JNK inhibitor-treated mice and the JNK1 knockout mice. Smoke exposure did not alter the expression of MUC1 and MUC4 proteins in all 3 groups compared with control.
Conclusion: An increase in epithelial MUC5AC protein expression is associated with the overproduction of mucus in smoke inhalation injury, and that its expression is related on JNK1 signaling.
Keimyung Author(s)(Kor)
최원일
권건영
Publisher
School of Medicine
Citation
Won-Il Choi et al. (2010). JNK activation is responsible for mucus overproduction in smoke inhalation injury. Respiratory Research, 11(1), 172–178. doi: 10.1186/1465-9921-11-172
Type
Article
ISSN
1465-993X
DOI
10.1186/1465-9921-11-172
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34342
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.