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Apoptosis by Cyclosporine in Mesangial Cells

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Author(s)
S.Y. HanE.J. ChangH.J. ChoiC.S. KwakS.B. ParkH.C. KimK.C. Mun
Keimyung Author(s)
Han, Seung YeupPark, Sung BaeKim, Hyun ChulKwak, Chun SikMun, Kyo Cheol
Department
Dept. of Internal Medicine (내과학)
Dept. of Biochemistry (생화학)
Kidney Institute (신장연구소)
Journal Title
Tansplantation Proceedings
Issued Date
2006
Volume
38
Issue
7
Abstract
Introduction. The immunosuppressive agent cyclosporine (CsA) is widely used to treat
allograft rejection and various autoimmune disorders. A major limiting factor in the use of
CsA is chronic nephrotoxicity. The pathogenesis of CsA-induced nephrotoxicity is not fully
understood. Several recent studies have suggested that CsA treatment directly induces
apoptosis in several cell types. The present study was undertaken to investigate the effects
of CsA on apoptosis of cultured rat mesangial cells (RMCs).
Methods. RMCs were treated with CsA at concentrations of 0.1 to 40 mol/L. Cell
viability was determined by MTT assay. Apoptotic protein expression was determined by
Western blot analysis.
Results. Cell viability was decreased with increasing concentrations of CsA in dosedependent
manner. CsA produced dose-dependent induction of p53, caspase-6, and Bax
protein expression. CsA treatment caused proteolytic cleavage of caspase-3 and induced
the degradation of 116-kDa PARP into 89-kDa fragment. RMCs with CsA reduced Bcl-2
and cIAP expression.
Conclusions. In this study, CsA induced apoptosis by up-regulating proapoptotic
factors, caspase-3 and -6, p53, Bax, cleaving PARP, and down-regulating antiapoptotic
factor, Bcl-2, and cIAP. These results suggested that the increased cell apoptosis exerted
by CsA may be one of the mechanisms promoting CsA-induced nephrotoxicity.
Keimyung Author(s)(Kor)
한승엽
박성배
김현철
곽춘식
문교철
Publisher
School of Medicine
Citation
S.Y. Han et al. (2006). Apoptosis by Cyclosporine in Mesangial Cells. Tansplantation Proceedings, 38(7), 2244–2246. doi: 10.1016/j.transproceed.2006.06.029
Type
Article
ISSN
0041-1345
Source
https://www.sciencedirect.com/science/article/pii/S0041134506006476?via%3Dihub
DOI
10.1016/j.transproceed.2006.06.029
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34456
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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