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“Universal” vitrification of cells by ultra-fast cooling

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Author(s)
Yun Seok HeoSunitha NagrathAlessandra L. MooreMahnaz ZeinaliDaniel IrimiaShannon L. StottThomas L. TothMehmet Toner
Keimyung Author(s)
Heo, Yun Seok
Department
Dept. of Biomedical Engineering (의용공학과)
Journal Title
Technology
Issued Date
2015
Volume
3
Issue
1
Abstract
Long-term preservation of live cells is critical for a broad range of clinical and research applications. With the increasing diversity of cells that need to be preserved (e.g. oocytes, stem and other primary cells, genetically modified cells), careful optimization of preservation protocols becomes tedious and poses significant limitations for all but the most expert users. To address the challenge of long-term storage of critical, heterogeneous cell types, we propose a universal protocol for cell vitrification that is independent of cell phenotype and uses only low concentrations of cryoprotectant (1.5 M PROH and 0.5 M trehalose). We employed industrial grade microcapillaries made of highly conductive fused silica, which are commonly used for analytical chemistry applications. The minimal mass and thermal inertia of the microcapillaries enabled us to achieve ultrafast cooling rates up to 4,000 K/s. Using the same low, non-toxic concentration of cryoprotectant, we demonstrate high recovery and viability rates after vitrification for human mammary epithelial cells, rat hepatocytes, tumor cells from pleural effusions, and multiple cancer cell lines.
Keimyung Author(s)(Kor)
허윤석
Publisher
School of Medicine
Citation
Yun Seok Heo et al. (2015). “Universal” vitrification of cells by ultra-fast cooling. Technology, 3(1), 64–71. doi: 10.1142/S2339547815500053
Type
Article
ISSN
2339-5478
DOI
10.1142/S2339547815500053
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34457
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biomedical Engineering (의용공학과)
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