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Melatonin Plays a Role as a Mediator of Nocturnal Pain in Patients with Shoulder Disorders

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Author(s)
Eunyoung HaYun-Mee LhoHyuk-Jun SeoChul-Hyun Cho
Keimyung Author(s)
Ha, Eun YoungCho, Chul Hyun
Department
Dept. of Biochemistry (생화학)
Dept. of Orthopedic Surgery (정형외과학)
Journal Title
Journal of bone and joint surgery. American Volume.
Issued Date
2014
Volume
96
Issue
13
Abstract
Background: Nocturnal pain is commonly observed in patients with shoulder disorders such as a rotator cuff tear or
frozen shoulder. This study was conducted to explore the possibility that melatonin plays a role as a mediator of nocturnal
pain in patients with a rotator cuff tear or frozen shoulder.
Methods: Subacromial bursa and joint capsule samples were collected from sixty-three patients: twenty-one patients
with a rotator cuff tear, twenty-two with frozen shoulder, and twenty with shoulder instability (control group). The expression
of melatonin receptor 1A (MTNR1A) and 1B (MTNR1B) and of acid-sensing ion channel 3 (ASIC3) in the subacromial bursa
and the joint capsule were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. The
protein level of ASIC3 was measured by immunoblot analysis. To determine the effect of melatonin as a pain mediator, an
in vitro study with use of primary cultured fibroblast-like synoviocytes was performed by semiquantitative RT-PCR analysis,
immunoblot analysis, and enzyme-linked immunosorbent assay (ELISA).
Results: MTNR1A, MTNR1B, and ASIC3 expression was significantly increased in both the rotator cuff tear and frozen
shoulder groups compared with the control group of patients with shoulder instability. Interleukin-1b (IL-1b) and tumor
necrosis factor-a (TNF-a) significantly stimulated the expression of MTNR1A and MTNR1B in primary cultured fibroblastlike
synoviocytes treated with proinflammatory cytokines. Melatonin treatment at a physiological concentration (10 nM)
induced ASIC3 expression and IL-6 production. Treatment with luzindole, amelatonin-receptor antagonist, reversedmelatoninstimulated
ASIC3 expression and IL-6 production.
Conclusions: Our study suggests that melatonin may play a role as a mediator of nocturnal pain with a rotator cuff tear or
frozen shoulder, and this effect may be mediated via melatonin receptors.
Clinical Relevance: Melatonin may be a therapeutic target of chronotherapy.
Keimyung Author(s)(Kor)
하은영
조철현
Publisher
School of Medicine
Citation
Eunyoung Ha et al. (2014). Melatonin Plays a Role as a Mediator of Nocturnal Pain in Patients with Shoulder Disorders. Journal of bone and joint surgery. American Volume., 96(13), e108(1)-e108(6).
Type
Article
ISSN
0021-9355
Source
https://insights.ovid.com/crossref?an=00004623-201407020-00010
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34492
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학)
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