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Augmentation of pulmonary host defense against Pseudomonas by FcγRIIA cDNA transfer to the respiratory epithelium

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Author(s)
Stefan WorgallPetr BezdicekMoo-Kyung KimJong-Gu ParkRavi SinghMelpo Christofidou-SolomidouAlice PrinceImre KovesdiAlan D. SchreiberRonald G. Crystal
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Journal of Clinical Investigation
Issued Date
1999
Volume
104
Issue
4
Abstract
Fcg receptors on the surface of phagocytic cells bind the Fc region of IgG and mediate binding, phagocytosis,
and destruction of particulate antigens opsonized by the antigen-specific IgG molecule. The
present study evaluates the feasibility of converting lung epithelial cells into phagocytic cells using
adenovirus (Ad) vector–mediated gene transfer of FcgRIIA cDNA to induce expression of the human
FcgRIIA receptor. Binding and phagocytosis of opsonized sheep red blood cells (SRBCs) by the A549
human lung epithelial cell line after Ad-mediated FcgRIIA gene transfer was demonstrated using light
and fluorescence microscopy and phagocytic assays with 51Cr-labeled SRBCs. When A549 cells were
infected with an Ad vector expressing a FcgRIIA mutant in which 2 of 3 cytoplasmic tyrosines have been
replaced with phenylalanine, only binding, but not phagocytosis, of opsonized SRBCs was observed. In
vivo expression of FcgRIIA in the lung after intratracheal administration of the AdFcgRIIA enhanced
clearance of opsonized Pseudomonas aeruginosa from the lung in normal rats and in mice deficient in Fcg
receptor expression. Similar results were observed with a chimeric FcgRIIA construct containing the
extracellular domain of FcgRIIIA. Together, these data demonstrate that Ad-mediated FcgRIIA receptor
cDNA expression can mediate the binding and phagocytosis of opsonized particulate antigens by
normally nonphagocytic cells, suggesting that gene-transfer strategies might be used to utilize nonphagocytic
cells to clear bacteria or other opsonized particulate antigens from the respiratory tract.
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
Stefan Worgall et al. (1999). Augmentation of pulmonary host defense against Pseudomonas by FcγRIIA cDNA transfer to the respiratory epithelium. Journal of Clinical Investigation, 104(4), 409–418. doi: 10.1172/JCI5432
Type
Article
ISSN
0021-9738
DOI
10.1172/JCI5432
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34496
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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