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Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea

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Author(s)
Jong Hwa YumEun Young LeeSung-Ho HurSeok Hoon JeongHyukmin LeeDongeun YongYunsop ChongEun-Woo LeePatrice NordmannKyungwon Lee
Keimyung Author(s)
Hur, Seung Ho
Department
Dept. of Internal Medicine (내과학)
Journal Title
Journal of Microbiology
Issued Date
2010
Volume
48
Issue
3
Keyword
E. meningosepticametallo-β-lactamaseGOBBlaBcluster
Abstract
This study was performed to characterize the chromosomal metallo-􀁅-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5􀄮. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the blaGOB genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of blaGOB gene, including 10 novel variants (blaGOB-8 to blaGOB-17). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory
concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5􀁄. BlaB and GOB MBLs were grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities.
Keywords: E. meningoseptica, metallo-􀁅-lactamase, GOB, BlaB, cluster
Keimyung Author(s)(Kor)
허승호
Publisher
School of Medicine
Citation
Jong Hwa Yum et al. (2010). Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea. Journal of Microbiology, 48(3), 358–364. doi: 10.1007/s12275-010-9308-5
Type
Article
ISSN
1225-8873
Source
https://link.springer.com/article/10.1007%2Fs12275-010-9308-5
DOI
10.1007/s12275-010-9308-5
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34503
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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