계명대학교 의학도서관 Repository

Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

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Author(s)
Dong-Ja KimSarah LinnstaedtJaime PalmaJoon Cheol ParkEvangelos NtrivalasJoanne Y.H. Kwak-KimAlice Gilman-SachsKenneth BeamanMichelle L. HastingsJeffrey N. MartinDominik M. Duelli
Keimyung Author(s)
Park, Joon Cheol
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Journal Title
Journal of Molecular Diagnostics Bimonthly
Issued Date
2012
Volume
14
Issue
1
Abstract
Circulating microRNAs (miRNAs) have emerged as
candidate biomarkers of various diseases and conditions
including malignancy and pregnancy. This approach
requires sensitive and accurate quantitation of
miRNA concentrations in body fluids. Herein we report
that enzyme-based miRNA quantitation, which is
currently the mainstream approach for identifying
differences in miRNA abundance among samples, is
skewed by endogenous serum factors that co-purify
with miRNAs and anticoagulant agents used during
collection. Of importance, different miRNAs were affected
to varying extent among patient samples. By
developing measures to overcome these interfering
activities, we increased the accuracy, and improved
the sensitivity of miRNA detection up to 30-fold. Overall,
the present study outlines key factors that prevent
accurate miRNA quantitation in body fluids and provides
approaches that enable faithful quantitation of
miRNA abundance in body fluids.
Keimyung Author(s)(Kor)
박준철
Publisher
School of Medicine
Citation
Dong-Ja Kim et al. (2012). Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation. Journal of Molecular Diagnostics Bimonthly, 14(1), 71–80. doi: 10.1016/j.jmol2011.09.002
Type
Article
ISSN
1525-1578
DOI
10.1016/j.jmol2011.09.002
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34505
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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