The Effects of Extrahepatic Cholestasis on Serum a-D-Mannosidase Isozyme Activities in Ethanol Intoxicated Rats

Abstract

Serum a-D-mannosidase isozyme activities were measured in rats with ethanol intoxication combined with extrahepatic cholestasis induced by common bile duct ligation for the manifestation of the biochemeical background of drinking hazards under the hepatobiliary disease. When chronic ethanol intoxication was combined with extrahepatic cholestasis, the activities of the rat's serum cytosolic, lysosomal and Golgi a-D-mannosidase isozymes increased at a more significant rate than those of the cholestasis alone. However, when acute ethanol intoxication was combined with extrahepatic cholestasis, the activities of the above isozymes were seen in the cholestasis alone. The results suggested that the elevated activities of these isozymes in chronic ethanol intoxication with cholestasis rather than in cholestasis alone were indications of increased hepatic damages, which caused these isozymes to leak into the blood in great quantity. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.

Key Words: Common bile duct ligation, Ethanol intoxication, Extrahepatic cholestasis, a-D-Mannosidase