계명대학교 의학도서관 Repository

Evaluation of the Usefulness of Selective Chromogenic Agar Medium (ChromID VRE) and Multiplex PCR Method for the Detection of Vancomycin-resistant Enterococci

Metadata Downloads
Author(s)
Do-Hoon KimJae-Hee LeeJung-Sook HaNam-Hee RyooDong-Seok JeonJae-Ryong Kim
Keimyung Author(s)
Ha, Jung SookRyoo, Nam HeeJeon, Dong SeokKim, Jae RyongKim, Do Hoon
Department
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
Korean Journal of Laboratory Medicine
Issued Date
2010
Volume
30
Issue
6
Keyword
VREChromogenic agarPCR
Abstract
Background

Accurate and early detection of vancomycin-resistant enterococci (VRE) is critical for controlling nosocomial infection. In this study, we evaluated the usefulness of a selective chromogenic agar medium and of multiplex PCR for detection of VRE, and both these techniques were compared with the conventional culture method for VRE detection.


Methods

We performed the following 3 methods for detecting VRE infection in stool specimens: the routine culture method, culturing in selective chromogenic agar medium (chromID VRE, bioMérieux, France), and multiplex PCR using the Seeplex® VRE ACE Detection kit (Seegene Inc., Korea) with additional PCR for vanC genes.


Results

We isolated 109 VRE strains from 100 stool specimens by the routine culture method. In chromID VRE, all the isolates showed purple colonies, including Enterococcus gallinarum and E. raffinosus, which were later identified using the Vitek card. All VRE isolates were identified by the multiplex PCR method; 100 were vanA-positive E. faecium, 8 were vanA- and vanC-1-positive E. gallinarum, and 1 was vanA-positive E. raffinosus.


Conclusions

For VRE surveillance, culturing the isolates in chromID VRE after broth enrichment appears to be an accurate, rapid, and easy method for routine screening test. Multiplex PCR is relatively expensive and needs skilled techniques for detecting VRE, but it can be an auxiliary tool for rapid detection of genotype during a VRE outbreak.

Keywords: VRE; Chromogenic agar; PCR
Keimyung Author(s)(Kor)
하정숙
류남희
전동석
김재룡
김도훈
Publisher
School of Medicine
Citation
Do-Hoon Kim et al. (2010). Evaluation of the Usefulness of Selective Chromogenic Agar Medium (ChromID VRE) and Multiplex PCR Method for the Detection of Vancomycin-resistant Enterococci. Korean Journal of Laboratory Medicine, 30(6), 631–636. doi: 10.3343/kjlm.2010.30.6.631
Type
Article
ISSN
1598-6535
DOI
10.3343/kjlm.2010.30.6.631
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34585
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.