계명대학교 의학도서관 Repository

Induction of COX-2 in human airway cells by manganese: Role of PI3K/PKB, p38 MAPK, PKCs, Src, and glutathione depletion

Metadata Downloads
Author(s)
Byeong-Churl Jang
Keimyung Author(s)
Jang, Byeong Churl
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Toxicol In Vitro
Issued Date
2009
Volume
23
Issue
1
Keyword
ManganeseCOX-2PI3K/PKBp38 MAPKPKCsSrcGSHA549 cells
Abstract
The exposure of manganese is believed to be the risk of respiratory diseases. COX-2 is a protein involved
in biosynthesis of inflammatory prostaglandins. Evidence suggests that COX-2 involves in the pathogen-
esis of lung inflammation. In this study, the effect of manganese–chloride (manganese) on COX-2 expres-
sion in A549 human lung epithelial cells was investigated. Treatment with manganese induced COX-2 at
both protein and mRNA levels that were due to COX-2 transcriptional activation. Interestingly, manga-
nese treatment led to activation of ERKs, p38 MAPK, JNKs, ATF-2, and PKB, but not NF-jB, and also cel-
lular GSH depletion in A549 cells. Importantly, the manganese-induced COX-2 expression was
suppressed by treatment with the inhibitor of p38 MAPK (SB203580), PI3K/PKB (LY294002), PKCs
(GO6983, GF109203X, Rottlerin), Src (PP1), or the thiol-containing compound (NAC). There was crosstalk
between p38 MAPK and GSH depletion or Src in response to manganese signal. Induction of COX-2 by
manganese was also seen in different human airway cells, including H292 (bronchial) or Hep2 (laryn-
geal). These results collectively suggest that manganese induces COX-2 by transcriptional up-regulation
in human airway cells and the induction appears to be cooperatively mediated via multiple signaling
pathways and GSH depletion.
2008 Elsevier Ltd. All rights reserved. Keywords:
Manganese
COX-2
PI3K/PKB
p38 MAPK
PKCs
Src
GSH
A549 cells
Keimyung Author(s)(Kor)
장병철
Publisher
School of Medicine
Citation
Byeong-Churl Jang. (2009). Induction of COX-2 in human airway cells by manganese: Role of PI3K/PKB, p38 MAPK, PKCs, Src, and glutathione depletion. Toxicol In Vitro, 23(1), 120–126. doi: 10.1016/j.tiv.2008.11.005
Type
Article
ISSN
0887-2333
Source
http://lps3.linkinghub.elsevier.com.proxy.dsmc.or.kr/retrieve/pii/S0887-2333(08)00275-0
DOI
10.1016/j.tiv.2008.11.005
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34670
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.