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Phlorofucofuroeckol A inhibits the LPS-stimulated iNOS and COX-2 expressions in macrophages via inhibition of NF-κB, Akt, and p38 MAPK

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Author(s)
A.-Reum KimMin-Sup LeeTai-Sun ShinHong HuaByeong-Churl JangJae-Sue ChoiDae-Seok ByunTadanobu UtsukiDonald IngramHyeung-Rak Kim
Keimyung Author(s)
Jang, Byeong Churl
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Toxicol In Vitro
Issued Date
2011
Volume
25
Issue
8
Keyword
Phlorofucofuroeckol APhlorotanninAnti-inflammationEcklonia stoloniferaNF-κBRAW 264.7 cells
Abstract
We have recently reported that phlorofucofuroeckol A isolated from the edible brown algae Ecklonia
stolonifera showed potential antioxidative and anti-inflammatory properties in macrophage stimulated
by LPS treatments. In this study, we further investigated the pharmacological characteristic of phlorofu-
cofuroeckol A in regulations of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2
through regulatory and signaling pathways using LPS-treated RAW 264.7 cells. Treatment with 20 lM
of phlorofucofuroeckol A significantly decreased levels of iNOS and COX-2 mRNA induced by LPS stimu-
lation. As results, levels of pro-inflammatory cytokines such as interleukin (IL)-1b, IL-6 and tumor necro-
sis factor (TNF)-a were significantly reduced by treatments of phlorofucofuroeckol A in LPS-stimulated
RAW 264.7 cells. Phlorofucofuroeckol A inhibited promoter activities of inflammatory-mediators (iNOS
and COX-2) and transcriptional factors (nuclear factor-jB, NF-jB, and AP-1) in LPS-treated RAW 264.7
cells. Moreover, phlorofucofuroeckol A inhibited activation of Akt and p38 MAPK in LPS-treated RAW
264.7 cells. These results indicate that the phlorofucofuroeckol A regulates iNOS and COX-2 expressions
through the NF-jB-dependent transcriptional control associated with inhibition of multiple signaling
proteins, suggesting potential candidates of phloroglucinol derivatives for treatments of inflammatory
diseases.
2011 Elsevier Ltd. All rights reserved Keywords:
Phlorofucofuroeckol A
Phlorotannin
Anti-inflammation
Ecklonia stolonifera
NF-jB
RAW 264.7 cells
Keimyung Author(s)(Kor)
장병철
Publisher
School of Medicine
Citation
A.-Reum Kim et al. (2011). Phlorofucofuroeckol A inhibits the LPS-stimulated iNOS and COX-2 expressions in macrophages via inhibition of NF-κB, Akt, and p38 MAPK. Toxicol In Vitro, 25(8), 1789–1795. doi: 10.1016/j.tiv.2011.09.012
Type
Article
ISSN
0887-2333
Source
http://lps3.linkinghub.elsevier.com.proxy.dsmc.or.kr/retrieve/pii/S0887-2333(11)00254-2
DOI
10.1016/j.tiv.2011.09.012
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34672
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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