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Effect of Tacrolimus on the Production of Oxygen Free Radicals in Hepatic Mitochondria

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Author(s)
S.Y. HanE.J. ChangH.J. ChoiC.S. KwakS.I. SuhJ.H. BaeS.B. ParkH.C. KimK.C. Mun
Keimyung Author(s)
Suh, Seong IlBae, Jae HoonHan, Seung YeupPark, Sung BaeKim, Hyun ChulKwak, Chun SikMun, Kyo Cheol
Department
Dept. of Microbiology (미생물학)
Dept. of Physiology (생리학)
Dept. of Internal Medicine (내과학)
Dept. of Biochemistry (생화학)
Kidney Institute (신장연구소)
Journal Title
Transplantation Proceedings
Issued Date
2006
Volume
38
Issue
7
Abstract
Objectives. Cyclosporine (CsA) causes side effects that occur mainly in the kidney but
also in the liver. Several reports have strongly suggested that the production of oxygen free
radicals (OFRs) is a common mechanism of CsA toxicity. However, tacrolimus is believed
to suppress the production of OFRs.
Methods. We obtained the mitochondrial fraction with 96% purity from rat liver using
a sucrose density gradient solution. Zero to 100 mol/L tacrolimus was incubated with the
mitochondrial fraction for 6 hours at 37°C. OFRs were evaluated by measuring the
fluorescent product from the oxidation of an oxidant-sensitive 2,7-dichlorefluorescein
using a VICTOR3 multilabel counter.
Results. The fluorescence units for OFR production were increased as the time of
exposure to tacrolimus passed from 1 to 6 hours. The fluorescence units in 0.1 mol/L
tacrolimus were 6.0 105
at 1 hour, 7.8 105
at 2 hours, 9.0 105
at 3 hours, 10.0 105
at 4 hours, 11.1 105
at 5 hours, and 11.4 105
at 6 hours. However, the fluorescence
units were similar although the tacrolimus concentration increases from 0.1 to 100 mol/L.
Conclusions. The results in this experiment suggested that tacrolimus induced the
production of OFRs depending on the exposure time.
Keimyung Author(s)(Kor)
서성일
배재훈
한승엽
박성배
김현철
곽춘식
문교철
Publisher
School of Medicine
Citation
S.Y. Han et al. (2006). Effect of Tacrolimus on the Production of Oxygen Free Radicals in Hepatic Mitochondria. Transplantation Proceedings, 38(7), 2242–2243. doi: 10.1016/j.transproceed.2006.06.033
Type
Article
ISSN
0041-1345
Source
https://www.sciencedirect.com/science/article/pii/S0041134506006518?via%3Dihub
DOI
10.1016/j.transproceed.2006.06.033
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34680
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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