계명대학교 의학도서관 Repository

Effects of Cyclosporine on Metalloproteinase in Endothelial Cells

Metadata Downloads
Author(s)
E. HaK.C. Mun
Keimyung Author(s)
Ha, Eun YoungMun, Kyo Cheol
Department
Dept. of Biochemistry (생화학)
Journal Title
Transplantation Proceedings
Issued Date
2012
Volume
44
Issue
4
Abstract
Objectives. Cyclosporine (CsA) is a potent agent widely used after organ transplanta-
tions and in treatment of various autoimmune disorders. Some patients suffer severe
complications including renal and vascular toxicity that are influenced by the degree of
endothelial damage. Dysregulation of metalloproteinase (MMP) activity is known to
contribute to renal and vascular diseases. To investigate the possible mechanisms of
posttransplantation complications in the kidney and vessels by CsA, we examined its effects
on metalloproteinases in endothelial cells using human umbilical vein endothelial cells
(HUVECs).
Methods. HUVECs isolated from umbilical cords by collagenase digestion were seeded
in 6-well plates at a density of 1 105
cells/well before treatment with 2–250 mol/L CsA
and a 24-hour incubation. Thereafter we performed gelatin zymography of MMP-1,
MMP-2, MMP-3, MMP-8, MMP-9, and MMp-13 to evaluate band density using a
luminescent image analyzer system with controls calculated as 100%.
Results. MMP-1, MMP-3, MMP-8, MMP-9, and MMP-13 activities were increased
after CsA treatment;MMP-1 121;MMP-3 164;MMP-8 133;MMP-9 124; and
MMP-13 121. In contrast, MMP-2 activity was decreased after CsA treatment;
MMP-2 79.
Conclusions. This study showed CsA to activate most MMPs (except MMP-2) in
endothelial cells. This result suggests that CsA may dysregulate MMPs in endothelial cells.
Keimyung Author(s)(Kor)
하은영
문교철
Publisher
School of Medicine
Citation
E. Ha and K.C. Mun. (2012). Effects of Cyclosporine on Metalloproteinase in Endothelial Cells. Transplantation Proceedings, 44(4), 991–992. doi: 10.1016/j.transproceed.2012.03.015
Type
Article
ISSN
0041-1345
Source
https://www.sciencedirect.com/science/article/pii/S0041134512002357?via%3Dihub
DOI
10.1016/j.transproceed.2012.03.015
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34697
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
Authorize & License
  • Authorize공개
  • EmbargoForever
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.