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Effects of Cyclosporine on Oxidative Stress in Human Bronchial Epithelial Cells

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Author(s)
D.S. JeonE.Y. HaK.C. Mun
Keimyung Author(s)
Jeon, Dong SeokHa, Eun YoungMun, Kyo Cheol
Department
Dept. of Laboratory Medicine (진단검사의학)
Dept. of Biochemistry (생화학)
Institute for Medical Science (의과학연구소)
Journal Title
Transplantation Proceedings
Issued Date
2012
Volume
44
Issue
4
Abstract
Objectives. Some of the airway complications relate to the use of cyclosporine (CsA), a
potent agent widely used after organ transplantations. Several recent studies have
demonstrated CsA treatment to induce reactive oxygen species (ROS). The present study
was undertaken to investigate effects of CsA on production of ROS and antoxidant defense
of airway cells using the human bronchial epithelial cell line BEAS-2B.
Methods. We measured biological antioxidant potential (BAP), as well as ROS and
malondialdehyde levels in BEAS-2B cells after CsA treatment, using Free Radical
Analytical System 4 kits (Diacron, Grosseto, Italy). ROS production was expressed as Carr
Units as established by the manufacturer and BAP as mol/2 105
cells; malondialde-
hyde, by the thiobarbituric acid assay.
Results. ROS production was increased in the BEAS-2B cells after CsA treatment: 73.5
at 0 (controls); 82.5 at 10; 84.0 at 30; 86.0 at 50; and 93.0 Carr Unit/2 105
cells at 100
g/mL of CsA. The levels of BAP were 1821 at 0 (controls), 1698 at 10; 1653 at 30; 1366
at 50 g/mL; and 1391 at 100 g/mL. The levels of malondialdehyde were increased: 3.8
at 0 (controls); 3.4 at 10; 4.4 at 30; 4.2 at 50: and 5.0 nmol/106
cells at 100 g/mL.
Conclusions. Increased production of ROS and decreased BAP by CsA in BEAS-2B
cells may increase malondialdehyde levels by radical-induced damage.
Keimyung Author(s)(Kor)
전동석
하은영
문교철
Publisher
School of Medicine
Citation
D.S. Jeon et al. (2012). Effects of Cyclosporine on Oxidative Stress in Human Bronchial Epithelial Cells. Transplantation Proceedings, 44(4), 988–990. doi: 10.1016/j.transproceed.2012.01.091
Type
Article
ISSN
0041-1345
Source
https://www.sciencedirect.com/science/article/pii/S0041134512001261?via%3Dihub
DOI
10.1016/j.transproceed.2012.01.091
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34698
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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