Comparison of Canine Single and Sequential Bilateral Lung Transplant Model for Lung Preservation Study

Abstract

The purpose of this study was to compare canine sequential bilateral lung transplantation (SBLT) with left single lung transplant (LSLT) models. After 24 hours of lung preservation with low potassium/dextran glucose (LPDG) solution, seven SBLT procedures were performed using mongrel dogs. The comparative group including nine cases of LSLT. After completion of each transplant, the dogs were maintained on a ventilator for 3 hours. Thereafter serial hemodynamics were evaluated with recipients with chest X-ray, com- puted tomography, and lung perfusion scan checked at 2 hours after transplantation. Pathological evaluations were also performed. Five of seven SBLT successfully completed the whole assessment, all LSLT survived. In SBLT, pulmonary arterial pressure and pulmonary vascular resistance were significantly elevated, but recovered 3 hours after reperfusion. The ultrastructural aspects of alveolae and pulmonary artery endothelium showed a reversible mild injury 24 hours after lung preservation and reperfusion. The present study suggests that the canine SBLT model using LPDG solution provides an excellent preservation effect and is more physiological as the dog is completely dependent on the function of the transplanted lung.