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Increased expression of cyclin G1 in leiomyoma compared with normal myometrium

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Affiliated Author(s)
백원기차순도조치흠
Alternative Author(s)
Baek, Won KiCha, Soon DoCho, Chi Heum
Journal Title
American Journal of Obstetrics and Gynecology
ISSN
0002-9378
Issued Date
2003
Abstract
OBJECTIVE: The purpose of this study was to detect the expression of cyclin G1 in leiomyoma and to investigate
the alteration of its expression compared with normal myometrial tissue that was obtained from the
same patient.
STUDY DESIGN: With the use of Northern blot analysis, Western blot analysis, and immunohistochemistry,
we analyzed the expression of cyclin G1 in 24 patients who underwent hysterectomies.
RESULTS: We found that messenger RNA levels of cyclin G1 were elevated in human leiomyomas compared
with their adjacent normal myometrial tissues. Consistent with elevated messenger RNA levels, high
levels of cyclin G1 protein expression were detected by immunoblot analysis in all leiomyoma samples. Immunohistochemistry
revealed that cyclin G1 is located mainly in the nucleus in both normal myometrium and
leiomyoma. However, higher levels of cyclin G1 were apparent in tumor regions compared with adjacent normal
myometrial regions. In addition, we found the expression levels of other cyclins (A and E) and CDK2
were elevated in leiomyomas compared with normal myometrium. Because cyclin G1 is a transcriptional target
of the p53 tumor suppressor, we examined the p53 status of all eight leiomyoma samples and found no
p53 mutations.
CONCLUSION: These results suggest that cyclin G1 is frequently overexpressed in uterine leiomyoma in a
p53-independent manner and that this abnormality could be attributed to the severe proliferation of human
uterine leiomyomas. (Am J Obstet Gynecol 2003;188:634-9.)
Key words: Cyclin G1, uterine leiomyoma
Department
Dept. of Microbiology (미생물학)
Dept. of Obstetrics & Gynecology (산부인과학)
Publisher
School of Medicine
Citation
Won-Ki Baek et al. (2003). Increased expression of cyclin G1 in leiomyoma compared with normal myometrium. American Journal of Obstetrics and Gynecology, 188(3), 634–639. doi: 10.1067/mob.2003.140
Type
Article
ISSN
0002-9378
DOI
10.1067/mob.2003.140
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34907
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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