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Up-regulation of MicroRNA 146b is Associated with Myelofibrosis in Myeloproliferative Neoplasma

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Author(s)
Jung-Sook HaHye-Ra Jung
Keimyung Author(s)
Ha, Jung SookJung, Hye Ra
Department
Dept. of Laboratory Medicine (진단검사의학)
Dept. of Pathology (병리학)
Journal Title
Annals of Clinical & Laboratory Science
Issued Date
2015
Volume
45
Issue
3
Abstract
In this study, our goal was to evaluate whether the expressions of microRNA (miR)-150, miR-
146b, miR-31 and miR-95 demonstrate primary myelofibrosis (PMF) specificity, associations with fibrosis
grade, hematologic phenotypes, or myeloproliferative neoplasm (MPN)-associated mutations. A total of 51
formalin-fixed and paraffin-embedded bone marrow MPN samples, including 15 polycythemia vera (PV),
26 essential thrombocythemia (ET), and 10 PMF, and 24 normal controls were included. The expression
of microRNA (miRNA) was detected by quantitative real-time polymerase chain reaction using miRNA
specific primers. RNU6-2 was analyzed for all samples as endogenous control for relative quantification.
Information for fibrosis, hematologic parameters, Janus kinase 2 (JAK2) V617F, and calreticulin (CALR)
mutations was obtained from medical records. Significant increment of miR-146b was detected in PMF
compared to normal controls (P=0.008). Moreover, expression of miR-146b tended to increase according
to increment of fibrosis grade, and patients with myelofibrosis (MF) grade 3 showed significantly higher
expression than patients with MF 0 to 2 (P=0.022, 0.001 and 0.013, respectively) or normal controls
(P<0.001). The expression of miR-31 also showed tendency to increase following fibrosis and miR-150
showed up-regulated expression in ET (P=0.015) compared to normal control. There was no relationship
between miRNA expression and hematologic indices except miR-95 showed negative correlation with platelet
count (P=0.024). There was no significant correlation between miRNA expression and JAK2 V617F or
CALR mutation. Up-regulation of miR-146b could be used as a fibrosis-indicating marker and might be
helpful in the study of fibrotic mechanism in MPN, as well as other fibrotic diseases.
Key words: microRNAs, miR-146b, primary myelofibrosis, myeloproliferative neoplasm
Keimyung Author(s)(Kor)
하정숙
정혜라
Publisher
School of Medicine
Citation
Jung-Sook Ha and Hye-Ra Jung. (2015). Up-regulation of MicroRNA 146b is Associated with Myelofibrosis in Myeloproliferative Neoplasma. Annals of Clinical & Laboratory Science, 45(3), 308–14.
Type
Article
ISSN
0091-7370
Source
http://www.annclinlabsci.org/content/45/3/308.long
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34965
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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