계명대학교 의학도서관 Repository

Induction of apoptosis and inhibition of cyclooxygenase-2 expression by N-methyl-N'-nitro-N-nitrosoguanidine in human leukemia cells

Metadata Downloads
Author(s)
Park, CheolChoi, Byung TaeKang, Kwang IlKwon, Taeg KyuCheong, JaehunLee, Won HoKim, Nam DeukChoi, Yung Hyun
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Anticancer Drugs
Issued Date
2005
Volume
16
Issue
5
Abstract
Previous studies have demonstrated that N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a well-known DNA alkylating agent, induces G2/M arrest and apoptotic cell death in several human cancer cell lines. In the present study, we investigated the effects of MNNG on the growth of a U937 human leukemia cell model. The effects of this compound were also tested on cyclooxygenase (COX) activity. Treatment of U937 cells with MNNG resulted in the inhibition of viability and the induction of apoptosis in a concentration-dependent manner, which was associated with a dose-dependent upregulation in pro-apoptotic Bax protein, downregulation of anti-apoptotic Bcl-2 and Bcl-xL proteins, and proteolytic activation of caspase-3 protease. Furthermore, MNNG decreased the levels of COX-2 mRNA and protein expression without significant changes in the levels of COX-1, which was correlated with inactivation of the reporter construct of a COX-2 promoter and decrease in prostaglandin E2 synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of MNNG.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Park, Cheol et al. (2005). Induction of apoptosis and inhibition of cyclooxygenase-2 expression by N-methyl-N’-nitro-N-nitrosoguanidine in human leukemia cells. Anticancer Drugs, 16(5), 507–513.
Type
Article
ISSN
0959-4973
Source
http://insights.ovid.com/pubmed?pmid=15846116
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35052
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.