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Salinomycin Induces Apoptosis via Death Receptor-5 up-regulation in Cisplatin-resistant Ovarian Cancer Cells

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Affiliated Author(s)
신소진권상훈차순도조치흠
Alternative Author(s)
Shin, So JinKwon, Sang HoonCha, Soon DoCho, Chi Heum
Journal Title
Anticancer Research
ISSN
0250-7005
Issued Date
2013
Abstract
Background: Chemo-resistance to cisplatin-centered cancer therapy is a major obstacle to effective disease treatment. Recently, salinomycin was proven to be highly-effective for the elimination of cancer stem cells both in vitro and in vivo. The objective of the present study was to evaluate the anticancer properties of salinomycin in cisplatin-resistant ovarian cancer cells (A2780cis). Materials and Methods: The tetrazolium dye (MTT) assay was used to determine cell viability. Flow cytometric analysis was performed to analyze the effect on cell cycle and apoptosis. The expression of apoptosis-related proteins was evaluated by western blot analysis. Results: Cell viability was significantly reduced by salinomycin treatment in a dose-dependent manner. Flow cytometry showed an increase in sub-G1 phase cells. Salinomycin increased the expression of death receptor-5 (DR5), caspase-8 and Fas-associated protein with death domain (FADD). A decline in the expression of FLICE-like inhibitory protein (FLIP), activation of caspase-3 and increased poly ADP-ribose polymerase (PARP) cleavage, triggered apoptosis. Furthermore, annexin-V staining also revealed the apoptotic induction. Conclusion: These findings provide important insights regarding the activation of caspase-8 and DR5, to our knowledge, for the first time in salinomycin-treated cisplatin-resistant ovarian cancer and demonstrate that salinomycin could be a prominent anticancer agent.
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Institute for Cancer Research (암연구소)
Publisher
School of Medicine
Citation
BIDUR PARAJULI et al. (2013). Salinomycin Induces Apoptosis via Death Receptor-5 up-regulation in Cisplatin-resistant Ovarian Cancer Cells. Anticancer Research, 33(4), 1457–1462.
Type
Article
ISSN
0250-7005
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35061
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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