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The novel phospholipase C activator, m-3M3FBS, induces apoptosis in tumor cells through caspase activation, down-regulation of XIAP and intracellular calcium signaling

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Author(s)
Eun Mi JungTae-Jin LeeJong-Wook ParkYoe-Sik BaeSang Hyun KimYung Hyun ChoiTaeg Kyu Kwon
Keimyung Author(s)
Park, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Institute for Medical Science (의과학연구소)
Journal Title
Apoptosis
Issued Date
2008
Volume
13
Issue
2
Abstract
We investigated the effect of the novel
phospholipase C activator, m-3M3FBS, on the apoptosis of
human renal Caki cancer cells. Treatment with m-3M3FBS
induced apoptosis of Caki cells, which was accompanied
by accumulation of sub-G1 phase and DNA fragmentation.
We found that induction of apoptosis is a common
response of several cancer cell types to m-3M3FBS treatment.
Overexpression of Bcl-2 and c-FLIPs fails to block
m-3M3FBS-induced apoptosis. However, ectopic expression
of XIAP partly inhibits m-3M3FBS-induced apoptosis
in Caki cells. m-3M3FBS-induced apoptosis appeared to
involve the XIAP down-regulation and caspase activation.
m-3M3FBS also induced the expression of a potential
proapoptotic gene, C/EBP homologous protein (CHOP),
however, suppression of CHOP expression by small
interfering RNA did not abrogate the m-3M3FBS-induced
apoptosis. In addition, inhibition of phospholipase C (PLC)
or chelation of intracellular calcium prevented m-3M3FBSinduced
apoptosis in Caki cells, suggesting that the
involvement of PLC pathway and intracellular calcium
signaling on the apoptosis in m-3M3FBS-treated Caki
cells. Collectively, our present results suggest that
m-3M3FBS-induced apoptosis in Caki cells may result
from the activation of caspase, down-regulation of XIAP
and intracellular Ca2+ release pathway and that m-3M3FBS
treatment might overcome the anti-apoptotic effect of Bcl-2
or c-FLIPs in cancer cells.
Keywords Phospholipase C activator m-3M3FBS
Apoptosis Calcium XIAP CHOP
Keimyung Author(s)(Kor)
박종욱
권택규
Publisher
School of Medicine
Citation
Eun Mi Jung et al. (2008). The novel phospholipase C activator, m-3M3FBS, induces apoptosis in tumor cells through caspase activation, down-regulation of XIAP and intracellular calcium signaling. Apoptosis, 13(2), 133–145. doi: 10.1007/s10495-007-0159-4
Type
Article
ISSN
1360-8186
Source
https://link.springer.com/article/10.1007%2Fs10495-007-0159-4
DOI
10.1007/s10495-007-0159-4
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35075
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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