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D-Glucosamine down-regulates HIF-1α through inhibition of protein translation in DU145 prostate cancer cells

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Author(s)
Jee-Young ParkJong-Wook ParkSeong-Il SuhWon-Ki Baek
Keimyung Author(s)
Park, Jong WookSuh, Seong IlBaek, Won Ki
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Journal Title
Biochemical and Biophysical Research Communications
Issued Date
2009
Volume
382
Issue
1
Abstract
d-Glucosamine has been reported to inhibit proliferation of cancer cells in culture and in vivo. In this study we report a novel response to d-glucosamine involving the translation regulation of hypoxia inducible factor (HIF)-1α expression. d-Glucosamine caused a decreased expression of HIF-1α under normoxic and hypoxic conditions without affecting HIF-1α mRNA expression in DU145 prostate cancer cells. d-Glucosamine inhibited HIF-1α accumulation induced by proteasome inhibitor MG132 and prolyl hydroxylase inhibitor DMOG suggesting d-glucosamine reduces HIF-1α protein expression through proteasome-independent pathway. Metabolic labeling assays indicated that d-glucosamine inhibits translation of HIF-1α protein. In addition, d-glucosamine inhibited HIF-1α expression induced by serum stimulation in parallel with inhibition of p70S6K suggesting d-glucosamine inhibits growth factor-induced HIF-1α expression, at least in part, through p70S6K inhibition. Taken together, these results suggest that d-glucosamine inhibits HIF-1α expression through inhibiting protein translation and provide new insight into a potential mechanism of the anticancer properties of d-glucosamine.

Keywords
d-Glucosamine;
Hif-1α;
VEGF;
p70S6K;
Prostate cancer
Keimyung Author(s)(Kor)
박종욱
서성일
백원기
Publisher
School of Medicine
Citation
Jee-Young Park et al. (2009). D-Glucosamine down-regulates HIF-1α through inhibition of protein
translation in DU145 prostate cancer cells. Biochemical and Biophysical Research Communications, 382(1), 96–101. doi: 10.1016/j.bbrc.2009.02.129
Type
Article
ISSN
0006-291X
Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X0900415X
DOI
10.1016/j.bbrc.2009.02.129
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35158
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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