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Tetradecanoyl phorbol acetate induces expression of Toll-like receptor 2 in U937 cells: involvement of PKC, ERK, and NF-κB

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Author(s)
Byeong-Churl JangTae-Young JungJi-Hye PaikYoung-Kyu KwonSang-Woo ShinSang-Pyo KimJung-Sook HaMin-Ho SuhSeong-Il Suh
Keimyung Author(s)
Jang, Byeong ChurlKim, Sang PyoHa, Jung SookSuh, Min HoSuh, Seong Il
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Pathology (병리학)
Dept. of Laboratory Medicine (진단검사의학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Journal Title
Biochemical and Biophysical Research Communications
Issued Date
2005
Volume
328
Issue
1
Abstract
Toll-like receptors (TLRs) have been identified recently as crucial signaling receptors mediating the innate immune recognition. Though induction of TLR2 or TLR4 by 12-O-tetradecanoyl phorbol 13-acetate (TPA) in leukemia cells has been reported, however, the mechanism by which TPA up-regulates TLR2 or TLR4 remains poorly understood. In this study, we investigated the effect of TPA on induction of TLR2 in U937 cells. TPA markedly induced TLR2 mRNA and protein expressions. TLR2 expression in response to TPA was attenuated by pretreatments with GF109203X and Go6976 (inhibitors of protein kinase C (PKC)) and PD98059 (an inhibitor of extracellular signal-regulated kinases (ERKs)), but not SB203580 (an inhibitor of p38s) and SP600125 (an inhibitor of c-Jun N-terminal kinases), suggesting involvement of PKC and ERKs in this response. Moreover, TPA-induced PKC activation was linked to generation of reactive oxygen species, which were dispensable for TLR2 expression in U937 cells. Pretreatments with GF109203X blocked TPA-induced phosphorylation of ERKs, suggesting activation of ERKs by PKC. In addition, TPA induced nuclear factor-κB (NF-κB) activation, which was shown by increased nuclear translocation of p65 NF-κB and degradation of IκB-α, a NF-κB inhibitory protein. Importantly, TPA-induced TLR2 expression was inhibited by blockage of NF-κB activation using NF-κB inhibitors, including MG132 and BAY11-7085. Specifically, TPA-induced nuclear translocation of NF-κB was effectively attenuated by GF109203X and PD98059, suggesting PKC and ERK regulation of NF-κB nuclear localization in response to TPA. Together, these results suggest that TPA-induced TLR2 expression in U937 cells may be at least in part mediated through activation of PKC and ERKs as well as NF-κB transcription factor, and that cross-talk between PKC or ERKs and NF-κB may exist.

Keywords
TPA;
TLR2;
PKC;
ERKs;
NF-κB
Keimyung Author(s)(Kor)
장병철
김상표
하정숙
서민호
서성일
Publisher
School of Medicine
Citation
Byeong-Churl Jang et al. (2005). Tetradecanoyl phorbol acetate induces expression of Toll-like receptor
2 in U937 cells: involvement of PKC, ERK, and NF-κB. Biochemical and Biophysical Research Communications, 328(1), 70–77. doi: 10.1016/j.bbrc.2004.12.144
Type
Article
ISSN
0006-291X
Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X04029584
DOI
10.1016/j.bbrc.2004.12.144
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35173
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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