계명대학교 의학도서관 Repository

Phospholipase D1 decreases type I collagen levels in hepatic stellate cells via induction of autophagy

Metadata Downloads
Author(s)
H.-Y. SeoB.-K. JangY.-A. JungE.-J. LeeH.-S. KimJ.-H. JeonJ.-G. KimI.-K. LeeM.-K. KimK.-G. Park
Keimyung Author(s)
Jang, Byoung KukKim, Hye SoonKim, Mi Kyung
Department
Dept. of Internal Medicine (내과학)
Journal Title
Biochemical and Biophysical Research Communications
Issued Date
2014
Volume
449
Issue
1
Abstract
Hepatic stellate cells (HSCs) are major players in liver fibrogenesis. Accumulating evidence shows that suppression of autophagy plays an important role in the development and progression of liver disease. Phospholipase D1 (PLD1), which catalyzes the hydrolysis of phosphatidylcholine to yield phosphatidic acid (PA) and choline, was recently shown to modulate autophagy. However, little is known about the effects of PLD1 on the production of type I collagen that characterizes liver fibrosis. Here, we examined whether PLD1 regulates type I collagen levels in HSCs through induction of autophagy. Adenovirus-mediated overexpression of PLD-1 (Ad-PLD1) reduced type I collagen levels in the activated human HSC lines, hTERT and LX2. Overexpression of PLD1 in HSCs led to induction of autophagy as demonstrated by increased LC3-II conversion and formation of LC3 puncta, and decreased p62 abundance. Moreover, inhibiting the induction of autophagy by treating cells with bafilomycin or a small interfering (si)RNA for ATG7 rescued Ad-PLD1-induced suppression of type I collagen accumulation in HSCs. The effects of PLD on type I collagen levels were not related to TGF-β/Smad signaling. Furthermore, treatment of cells with PA induced autophagy and inhibited type I collagen accumulation. The present study indicates that PLD1 plays a role in regulating type I collagen accumulation through induction of autophagy.Keywords
Hepatic stellate cells;
Phospholipase D1;
Autophagy
Keimyung Author(s)(Kor)
장병국
김혜순
김미경
Publisher
School of Medicine
Citation
H.-Y. Seo et al. (2014). Phospholipase D1 decreases type I collagen levels in hepatic stellate cells
via induction of autophagy. Biochemical and Biophysical Research Communications, 449(1), 38–43. doi: 10.1016/j.bbrc.2014.04.149
Type
Article
ISSN
0006-291X
Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X14008122
DOI
10.1016/j.bbrc.2014.04.149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35178
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.