계명대학교 의학도서관 Repository

Regulation of IGFBP-2 expression during fasting

Metadata Downloads
Author(s)
Hye Suk KangMi-Young KimSeung-Jae KimJae-Ho LeeYong-Deuk KimYoung-Kyo SeoJae-Hoon BaeGoo-Taeg OhDae-Kyu SongYong-Ho AhnSeung-Soon Im
Keimyung Author(s)
Lee, Jae HoBae, Jae HoonSong, Dae KyuIm, Seung Soon
Department
Dept. of Anatomy (해부학)
Dept. of Physiology (생리학)
Journal Title
Biochemical Journal
Issued Date
2015
Volume
467
Issue
3
Abstract
Insulin-like growth factor (IGF)-binding protein-2 (IGFBP-2),
one of the most abundant circulating IGFBPs, is known to
attenuate the biological action of IGF-1. Although the effect
of IGFBP-2 in preventing metabolic disorders is well known,
its regulatory mechanism remains unclear. In the present study,
we demonstrated the transcriptional regulation of the Igfbp-2
gene by peroxisome-proliferator-activated receptor (PPAR) α in
the liver. During fasting, both Igfbp-2 and PPARα expression
levels were increased. Wy14643, a selective PPARα agonist,
significantly induced Igfbp-2 gene expression in primary cultured
hepatocytes. However, Igfbp-2 gene expression in Pparα null
micewas not affected by fasting orWy14643. In addition, through
transient transfection and chromatin immunoprecipitation assay
in fasted livers, we determined that PPARα bound to the putative
PPAR-responsive element between −511 bp and −499 bp on the
Igfbp-2 gene promoter, indicating that the Igfbp-2 gene
transcription is activated directly by PPARα. To explore the
role of PPARα in IGF-1 signalling, we treated primary cultured
hepatocytes with Wy14643 and observed a decrease in the
number of IGF-1 receptors (IGF-1Rs) and in Akt phosphorylation.
No inhibition was observed in the hepatocytes isolated from
Pparα null mice. These results suggest that PPARα controls
IGF-1 signalling through the up-regulation of hepatic Igfbp-2
transcription during fasting and Wy14643 treatment.
Key words: fasting, gene expression, insulin-like growth factor-1
(IGF-1), insulin-like growth factor-binding protein 2 (IGFBP-2),
liver, peroxisome-proliferator-activated receptor α (PPARα).
Keimyung Author(s)(Kor)
이재호
배재훈
송대규
임승순
Publisher
School of Medicine
Citation
Hye Suk Kang et al. (2015). Regulation of IGFBP-2 expression during fasting. Biochemical Journal, 467(3), 453–460. doi: 10.1042/BJ20141248
Type
Article
ISSN
0264-6021
DOI
10.1042/BJ20141248
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35183
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.