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Quercetin arrests G2/M phase and induces caspase-dependent cell death in U937 cells

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Author(s)
Tae-Jin LeeOn Hee KimYeoun Hee KimJun Hee LimShin KimJong-Wook ParkTaeg Kyu Kwon
Keimyung Author(s)
Kim, ShinPark, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Institute for Medical Science (의과학연구소)
Journal Title
Cancer Letters
Issued Date
2006
Volume
240
Issue
2
Abstract
Quercetin, a natural product derived from grapes, has been shown to prevent carcinogenesis in murine models. We report here that quercetin induces anti-proliferation and arrests G2/M phase in U937 cells. The G2/M phase accumulation was accompanied by an increase in the level of the cyclin B. In contrast, the level of the cyclin D, cyclin E, E2F1, and E2F2 was marked decreased in quercetin-treated U937 cells. Removal of quercetin from the culture medium stimulates U937 cells to synchronously re-enter the cell cycle, decrease expression level of cyclin B, and increased the expression level of cyclin D and cyclin E. These data demonstrate that quercetin causes reversible G2/M phase arrest, which was related with dramatic changes in the level of cyclin B, cyclin D, and cyclin E. Quercetin-induced down-regulation of cyclin D and cyclin E was associated with suppression of transcriptional levels but not protein stability.

In addition, quercetin-treated U937 cells showed DNA fragmentation, increased sub-G1 population, and generated a 60 kDa cleavage product of PLC-γ1 in a dose-dependent manner, which were significantly inhibited by z-VAD-fmk. These data clearly indicate that quercetin-induced apoptosis is associated with caspase activation.

In summary, the growth inhibition of the quercetin is highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human promonocytic U937 cells.

Keywords
Quercetin;
G2/M arrest;
Caspase;
U937
Keimyung Author(s)(Kor)
김신
박종욱
권택규
Publisher
School of Medicine
Citation
Tae-Jin Lee et al. (2006). Quercetin arrests G2/M phase and induces caspase-dependent cell death in U937 cells. Cancer Letters, 240(2), 234–242. doi: 10.1016/j.canlet.2005.09.013
Type
Article
ISSN
0304-3835
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0304383505008669?via%3Dihub
DOI
10.1016/j.canlet.2005.09.013
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35324
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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