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Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts

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Author(s)
Do Sik MinTaeg Kyu KwonWon-Sang ParkJong-Soo ChangSeung-Kiel ParkBong-Hyun AhnZae-Young RyooYoung Han LeeYeon Soo LeeDuck-Joo RhieShin-Hee YoonSang June HahnMyung-Suk KimYang-Hyeok Jo
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Carcinogenesis
Issued Date
2001
Volume
22
Issue
10
Abstract
Phospholipase D (PLD) has been suggested to play an important role in a variety of cellular functions. PLD activity has been shown to be significantly elevated in many tumours and transformed cells, suggesting the possibility that PLD might be involved in tumorigenesis. In this study, we have established stable cell lines overexpressing PLD1 and PLD2 from fibroblast cells. These cells, but not control cells, showed altered growth properties and anchorage-independent growth in soft agar. Both PLD1 and PLD2 also induced an up-regulation of the activity of matrix metalloprotease-9 as detected by zymograms. Furthermore, both PLD1 and PLD2 transformants, but not vector-transfectants, induced undifferentiated sarcoma when transplanted into nude mice. Both PLD1- and PLD2-mediated cell cycle distributions in stable cell lines revealed an increased fraction of cells in the S phase compared with control cells. Interestingly, the level of cyclin D3 protein, known as an activator of G1 to S phase transition in the cell cycle, was aberrantly high in cells overexpressing PLD1 and PLD2 compared with control cells. These results suggest that overexpression of PLD isozymes may play an important role in neoplastic transformation.
cdk, cyclin-dependent kinase
LPA, lysophosphatidic acid
MMP, matrix metalloprotease
PA, phosphatidic acid
PLD, phospholipase D.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Do Sik Min et al. (2001). Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts. Carcinogenesis, 22(10), 1641–1647. doi: 10.1093/carcin/22.10.1641
Type
Article
ISSN
0143-3334
DOI
10.1093/carcin/22.10.1641
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35359
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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