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Cafestol, a coffee-specific diterpene, induces apoptosis in renal carcinoma Caki cells through down-regulation of anti-apoptotic proteins and Akt phosphorylation

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Author(s)
Min Jung ChoiEun Jung ParkJung Hwa OhKyoung-Jin MinEun Sun YangYoung Ho KimTae Jin LeeSang Hyun KimYung Hyun ChoiJong-Wook ParkTaeg Kyu Kwon
Keimyung Author(s)
Park, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Chemico-Biological Interactions
Issued Date
2011
Volume
190
Issue
2-3
Abstract
Cafestol, one of the major compounds in coffee beans, has been reported for its tumor cell growth inhibitory activity and anti-carcinogenic activity, although the mechanism of action is poorly understood. In the present study, we investigated the effect of cafestol on the apoptotic pathway in human renal Caki cells and other cancer cell lines. Cafestol treatment inhibited Caki cells viability a dose-dependent manner by inducing apoptosis, as evidenced by DNA fragmentation and the accumulation of sub-G1 phase. Cafestol-induced apoptosis is associated with the reduction of mitochondrial membrane potential (MMP), activation of caspase 3, cytochrome c release, and down-regulation of anti-apoptotic proteins (Bcl-2, Bcl-xL, Mcl-1 and cFLIP). Cafestol-induced apoptosis was blocked by pretreatment with broad caspase inhibitor z-VAD-fmk, showing its dependence on caspases. Ectopic expression of Bcl-2 or Mcl-1 in Caki cells attenuates cafestol-induced apoptosis. In addition, we have also shown that cafestol inhibits phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway, and PI3K inhibitor LY29004 significantly increases cafestol-induced apoptosis in Caki cells. Taken together, our results show the activity of cafestol to modulate multiple components in apoptotic response of human renal Caki cells and a potential as a therapeutic agent for preventing cancers such as renal carcinoma.

Keywords
Cafestol;
Apoptosis;
Akt;
Caki;
Bcl-2
Keimyung Author(s)(Kor)
박종욱
권택규
Publisher
School of Medicine
Citation
Min Jung Choi et al. (2011). Cafestol, a coffee-specific diterpene, induces apoptosis in renal carcinoma Caki cells through down-regulation of anti-apoptotic proteins and Akt phosphorylation. Chemico-Biological Interactions, 190(2–3), 102–108. doi: 10.1016/j.cbi.2011.02.013
Type
Article
ISSN
0009-2797
Source
https://www.sciencedirect.com/science/article/pii/S0009279711000718?via%3Dihub
DOI
10.1016/j.cbi.2011.02.013
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35391
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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