계명대학교 의학도서관 Repository

Silibinin induces apoptosis of HT29 colon carcinoma cells through early growth response-1 (EGR-1)-mediated non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) up-regulation

Metadata Downloads
Author(s)
Seon Min WooKyoung-jin MinShin KimJong-Wook ParkDong Eun KimKyung-Soo ChunYoung Ho KimTae-Jin LeeSang Hyun KimYung Hyun ChoiJong-Soo ChangTaeg Kyu Kwon
Keimyung Author(s)
Kim, ShinPark, Jong WookKwon, Taeg KyuKim, Dong Eun
Department
Dept. of Immunology (면역학)
Dept. of Otorhinolaryngology (이비인후과학)
Journal Title
Chemico-Biological Interactions
Issued Date
2014
Volume
211
Abstract
Silibinin, an effective anti-cancer and chemopreventive agent, has been shown to exert multiple effects on cancer cells, including inhibition of both cell proliferation and migration. However, the molecular mechanisms responsible for these effects are not fully understood. We observed that silibinin significantly induced the expression of the non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) in both p53 wild-type and p53-null cancer cell lines, suggesting that silibinin-induced NAG-1 up-regulation is p53-independent manner. Silibinin up-regulates early growth response-1 (EGR-1) expression. The ectopic expression of EGR-1 significantly increased NAG-1 promoter activity and NAG-1 protein expression in a dose-dependent manner. Furthermore, down-regulation of EGR-1 expression using siRNA markedly reduced silibinin-mediated NAG-1 expression, suggesting that the expression of EGR-1 is critical for silibinin-induced NAG-1 expression. We also observed that reactive oxygen species (ROS) are generated by silibinin; however, ROS did not affect silibinin-induced NAG-1 expression and apoptosis. In addition, we demonstrated that the mitogen-activated protein kinase (MAP kinase) signal transduction pathway is involved in silibinin-induced NAG-1 expression. Inhibitors of p38 MAP kinase (SB203580) attenuated silibinin-induced NAG-1 expression. Furthermore, we found that siRNA-mediated knockdown of NAG-1 attenuated silibinin-induced apoptosis. Collectively, the results of this study demonstrate for the first time that up-regulation of NAG-1 contributes to silibinin-induced apoptosis in cancer cells.

Keywords
Silibinin;
NAG-1;
Apoptosis;
EGR-1;
p53;
Colon carcinoma cells
Keimyung Author(s)(Kor)
김신
박종욱
권택규
김동은
Publisher
School of Medicine
Citation
Seon Min Woo et al. (2014). Silibinin induces apoptosis of HT29 colon carcinoma cells through early growth response-1 (EGR-1)-mediated non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) up-regulation. Chemico-Biological Interactions, 211, 36–43. doi: 10.1016/j.cbi.2014.01.004
Type
Article
ISSN
0009-2797
Source
https://www.sciencedirect.com/science/article/pii/S000927971400012X?via%3Dihub
DOI
10.1016/j.cbi.2014.01.004
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35392
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.