계명대학교 의학도서관 Repository

The coffee diterpene kahweol sensitizes TRAIL-induced apoptosis in renal carcinoma Caki cells through down-regulation of Bcl-2 and c-FLIP

Metadata Downloads
Author(s)
Hee Jung UmJung Hwa OhYoon-Nyun KimYung Hyun ChoiSang Hyun KimJong-Wook ParkTaeg Kyu Kwon
Keimyung Author(s)
Park, Jong WookKwon, Taeg KyuKim, Yoon Nyun
Department
Dept. of Immunology (면역학)
Dept. of Internal Medicine (내과학)
Journal Title
Chemico-biological interactions
Issued Date
2010
Volume
186
Issue
1
Abstract
Kahweol, a coffee-specific diterpene, found in the beans of Coffea arabica, has potent anti-carcinogenic, anti-tumor, and anti-inflammatory properties. TRAIL is a potential anti-cancer compound that induces apoptosis in a wide variety of cancer cells, but not in most normal human cell types. In the present study, we show that kahweol sensitizes human renal cancer cells, but not normal human mesangial cells, to TRAIL-mediated apoptosis. Moreover, treatment with a combination of kahweol and TRAIL induces significant apoptosis in various cancer cell types, thus presenting an attractive novel strategy for cancer treatment. Our experiments show that treatment with a combination of kahweol and TRAIL-induced apoptosis, and stimulated of DEVDase activity, DNA fragmentation, and cleavage of PARP, which was prevented by pretreatment with z-VAD, indicative of cell death via a caspase-dependent pathway. Kahweol-induced down-regulation of Bcl-2 and ectopic expression of Bcl-2 led to attenuation of kahweol plus TRAIL-mediated apoptosis, indicative of Bcl-2 involvement in the apoptotic process. In addition, the c-FLIP and caspase signal pathways seem to play a crucial role in apoptosis triggered by the combination of kahweol and TRAIL in Caki cells. Our results collectively demonstrate that down-regulation of Bcl-2 and c-FLIP contributes to the sensitizing effect of kahweol on TRAIL-mediated apoptosis in cancer cells.

Keywords
Kahweol;
TRAIL;
Apoptosis;
Caki;
Bcl-2;
c-FLIP
Keimyung Author(s)(Kor)
박종욱
권택규
김윤년
Publisher
School of Medicine
Citation
Hee Jung Um et al. (2010). The coffee diterpene kahweol sensitizes TRAIL-induced apoptosis in renal carcinoma Caki cells through down-regulation of Bcl-2 and c-FLIP. Chemico-biological interactions, 186(1), 36–42. doi: 10.1016/j.cbi.2010.04.013
Type
Article
ISSN
0009-2797
Source
https://www.sciencedirect.com/science/article/pii/S0009279710002991?via%3Dihub
DOI
10.1016/j.cbi.2010.04.013
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35395
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.