Anticancer Activity and Differentially Expressed Genes in Head and Neck Cancer Cells Treated with a Novel Cyclin-Dependent Kinase Inhibitor
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- 송달원; 권택규; 박종욱; 이성용; 백원기; 장병철
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- Song, Dal Won; Kwon, Taeg Kyu; Park, Jong Wook; Lee, Seong Ryong; Baek, Won Ki; Jang, Byeong Churl
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- Background: Cyclin-dependent kinases (CDKs) are involved in the regulation of the cell cycle and the growth of tumor cells. In this study, we investigated the antitumor effect and differentially expressed genes (DEGs) in head and neck cancer cells treated by a novel CDK inhibitor, 2-[1,1′-biphenyl]- 4-yl-N-[5-(1,1-dioxo-1λ6-isothiazolidin-2-yl)-1H-indazol-3-yl] acetamide (BAI). Methods: Cell growth was measured by XTT assay. Cell cycle and apoptosis were determined using flow cytometry. GeneFishing™ PCR was utilized to identify DEGs. Protein expression was analyzed by Western blot. Results: Exposure to BAI of 2 different head and neck cancer cell lines, AMC-HN4 and AMC-HN6, induced apoptosis in association with growth inhibition, cell cycle arrest, caspase-3 activation and cytochrome c release. Significantly, data from GeneFishing PCR experiments demonstrated 10 DEGs in AMC-HN6 cells treated with BAI. Some of these DEGs turned out to encode proteins with functions related to key cellular processes. Conclusions: These results indicate that BAI has strong anticancer activities on head and neck cancer cells, and the DEGs induced by BAI may become involved in BAI-induced cancer cell death.
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