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Gastrostomy in a Patient with Situs Inversus Totalis

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Author(s)
Hyung Ki LeeKwang Bum ChoEun Soo KimKyung Sik Park
Keimyung Author(s)
Cho, Kwang BumKim, Eun SooPark, Kyung Sik
Department
Dept. of Internal Medicine (내과학)
Journal Title
Clinical Endoscopy
Issued Date
2013
Volume
46
Issue
6
Abstract
Purpose: The addition of molecular targeted agents to enhance the cytotoxicity of chemotherapeutic agents is a promising strategy in cancer treatment. The combination of cyclooxygenase-2 inhibitors and epidermal growth factor receptor tyrosine kinase inhibitors, such as celecoxib and ZD1839 (gefitinib), was reported to achieve synergistic cell growth inhibition in squamous cell carcinoma of the head and neck. Therefore, we postulated that the addition of celecoxib and ZD1839 to docetaxel, a cytotoxic agent, might further increase antitumor activity.

Experimental Design: The combination of celecoxib, ZD1839, and docetaxel was studied for its effect on cell growth and apoptosis by cell growth inhibition and Annexin V assays. The relevant molecular targets of these agents and apoptotic markers were examined by immunoblotting analyses in the presence or absence of these three drugs. Morphologic changes of the microtubule cytoskeleton, a known target of docetaxel, were also evaluated by staining for α-tubulin after the combination treatment.

Results: We showed that this triple combination significantly enhanced cell growth inhibition and docetaxel-induced apoptosis. Docetaxel mainly induced caspase-8 activation, whereas the addition of celecoxib and ZD1839 augmented the caspase-8 activation and enhanced caspase-9 activation. One of the underlying mechanisms for augmentation of docetaxel-induced apoptosis by celecoxib and ZD1839 is to further inhibit the activation of prosurvival pathway molecules, such as extracellular signal-regulated kinase and AKT, and the promotion of aberrant apoptosis.

Conclusions: Our studies suggest that the combination of docetaxel with a cyclooxygenase-2 inhibitor and an epidermal growth factor receptor tyrosine kinase inhibitor may further improve efficacy of docetaxel and other taxane-based therapies in squamous cell carcinoma of the head and neck.
Keimyung Author(s)(Kor)
조광범
김은수
박경식
Publisher
School of Medicine
Citation
Hyung Ki Lee et al. (2013). Gastrostomy in a Patient with Situs Inversus Totalis. Clinical Endoscopy, 46(6), 662–665.
Type
Article
ISSN
2234-2400
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35446
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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