계명대학교 의학도서관 Repository

(–)-EPIGALLOCATECHIN GALLATE ATTENUATES GLUTAMATE-INDUCED CYTOTOXICITY VIA INTRACELLULAR CA2+ MODULATION IN PC12 CELLS

Metadata Downloads
Author(s)
Jong-Hun LeeDae-Kyu SongChul-Ho JungDong-Hoon ShinJongWook ParkTaeg Kyu KwonByeong-Churl JangKyo-Cheol MunSang-Pyo KimSeong-Il SuhJae Hoon Bae
Keimyung Author(s)
Jung, Chul HoSong, Dae KyuBae, Jae HoonKwon, Taeg KyuPark, Jong WookSuh, Seong IlKim, Sang PyoMun, Kyo CheolJang, Byeong ChurlShin, Dong Hoon
Department
Dept. of Psychiatry (정신건강의학)
Dept. of Physiology (생리학)
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Dept. of Pathology (병리학)
Dept. of Biochemistry (생화학)
Dept. of Molecular Medicine (분자의학)
Dept. of Preventive Medicine (예방의학)
Brain Research Institute (뇌연구소)
Journal Title
Clinical and Experimental Pharmacology and Physiology
Issued Date
2004
Volume
31
Issue
8
Abstract
SUMMARY
1. The effects of (–)-epigallocatechin gallate (EGCG), a
green tea polyphenol, on glutamate-induced increases in intracellular
Ca2+ concentrations ([Ca2+]i) and cytotoxicity in PC12
cells were investigated.
2. Changes in [Ca2+]i were measured using Fura-2/AM
calcium indicator dye and cellular viabilities were determined
by a viable cell count and a 3-(4,4-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide reduction assay.
3. Glutamate increased [Ca2+]i in PC12 cells in a dosedependent
manner. (–)-Epigallocatechin gallate attenuated this
glutamate (30 mmol/L)-induced [Ca2+]i increase and EGCG
(50
mol/L) increased the viability of PC12 cells against
glutamate-induced cytotoxicity. The EGCG effect was also
found to be independent of its general anti-oxidant mechanism.
In contrast, EGCG directly suppressed both N-methyl-Daspartate
(50 mmol/L)- and kainate (20 mmol/L)-mediated
Ca2+ influx, but not metabotropic receptor-mediated Ca2+
release.
4. These results suggest that EGCG reduces the glutamateinduced
[Ca2+]i increase by attenuating ionotropic Ca2+ influx
and that this promotes the viability of PC12 cells.
Key words: (–)-epigallocatechin gallate, glutamate, intracellular
calcium modulation.
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.