(–)-EPIGALLOCATECHIN GALLATE ATTENUATES GLUTAMATE-INDUCED CYTOTOXICITY VIA INTRACELLULAR CA2+ MODULATION IN PC12 CELLS
- Affiliated Author(s)
- 정철호; 송대규; 배재훈; 권택규; 박종욱; 서성일; 김상표; 문교철; 장병철; 신동훈
- Alternative Author(s)
- Jung, Chul Ho; Song, Dae Kyu; Bae, Jae Hoon; Kwon, Taeg Kyu; Park, Jong Wook; Suh, Seong Il; Kim, Sang Pyo; Mun, Kyo Cheol; Jang, Byeong Churl; Shin, Dong Hoon
- Journal Title
- Clinical and Experimental Pharmacology and Physiology
- Issued Date
1. The effects of (–)-epigallocatechin gallate (EGCG), a
green tea polyphenol, on glutamate-induced increases in intracellular
Ca2+ concentrations ([Ca2+]i) and cytotoxicity in PC12
cells were investigated.
2. Changes in [Ca2+]i were measured using Fura-2/AM
calcium indicator dye and cellular viabilities were determined
by a viable cell count and a 3-(4,4-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide reduction assay.
3. Glutamate increased [Ca2+]i in PC12 cells in a dosedependent
manner. (–)-Epigallocatechin gallate attenuated this
glutamate (30 mmol/L)-induced [Ca2+]i increase and EGCG
mol/L) increased the viability of PC12 cells against
glutamate-induced cytotoxicity. The EGCG effect was also
found to be independent of its general anti-oxidant mechanism.
In contrast, EGCG directly suppressed both N-methyl-Daspartate
(50 mmol/L)- and kainate (20 mmol/L)-mediated
Ca2+ influx, but not metabotropic receptor-mediated Ca2+
4. These results suggest that EGCG reduces the glutamateinduced
[Ca2+]i increase by attenuating ionotropic Ca2+ influx
and that this promotes the viability of PC12 cells.
Key words: (–)-epigallocatechin gallate, glutamate, intracellular
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