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INHIBITORY EFFECTS OF GLUCOSAMINE ON LIPOPOLYSACCHARIDE-INDUCED ACTIVATION IN MICROGLIAL CELLS

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Author(s)
Hyon-Ah YiSang-Doe YiByeong-Churl JangDae-Kyu SongDong-Hoon ShinKyo-Chul MunSang-Pyo KimSeong-Il SuhJae Hoon Bae
Keimyung Author(s)
Yi, Hyon AhYi, Sang DoSong, Dae KyuBae, Jae HoonShin, Dong HoonSuh, Seong IlKim, Sang PyoMun, Kyo CheolJang, Byeong Churl
Department
Dept. of Neurology (신경과학)
Dept. of Physiology (생리학)
Dept. of Preventive Medicine (예방의학)
Dept. of Microbiology (미생물학)
Dept. of Pathology (병리학)
Dept. of Biochemistry (생화학)
Dept. of Molecular Medicine (분자의학)
Brain Research Institute (뇌연구소)
Journal Title
Clinical and Experimental Pharmacology and Physiology
Issued Date
2005
Volume
32
Issue
12
Abstract
SUMMARY

1. The aim of the present study was to investigate the effects
of glucosamine on lipopolysaccharide (LPS)-induced cellular
activation in microglia and to evaluate the inhibitory
mechanisms involved.
2. Lipopolysaccharide (100 ng/mL) was used for the
activation of primary cultured rat microglial or BV2 microglial
cells. Changes in intracellular Ca

2+

levels and outward K

+

currents were measured using fura-2/AM and whole-cell
patch-clamp methods, respectively. Lipopolysaccharide-
induced expression of tumour necrosis factor (TNF)-



mRNA
was analysed by reverse transcription–polymerase chain
reaction.
3. Lipopolysaccharide transformed cell morphology into an
amoeboid shape

in



vitro

and induced microglial activation

in vivo

, as measured by immunohistochemical staining, but
glucosamine inhibited this activation. Glucosamine also
inhibited LPS-induced Ca

2+

influx, outward K

+

currents and
TNF-



mRNA expression, which are typically representative
of microglial activation.
4. The results suggest that the inhibitory mechanisms of
glucosamine on LPS-induced microglial activation include
inhibition of Ca

2+

influx and outward K

+

currents, as well as
downregulation of the microglial activator gene TNF-



.
Key words: glucosamine, lipopolysaccharide, microglial
activation.
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