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Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells

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Author(s)
Sung-Il NamTaeg-Kyu Kwon
Keimyung Author(s)
Nam, Sung IlKwon, Taeg Kyu
Department
Dept. of Otorhinolaryngology (이비인후과학)
Dept. of Immunology (면역학)
Journal Title
Clinical and Experimental Otorhinolaryngology
Issued Date
2014
Volume
7
Issue
3
Abstract
enObjectives.
To investigate the effect of interleukin (IL)-1β on matrix metalloproteinase (MMP)-9 expression in cochlea and regulation of IL-1β-mediated MMP-9 expression by dexamethasone and the molecular and signaling mechanisms involved.
Methods. House ear institute-organ of Corti 1 (HEI-OC1) cells were used and exposed to IL-1β with/without dexamethasone. Glucocorticoid receptor antagonist, RU486, was used to see the role of dexamethasone. PD98059 (an extracellular signal-regulated kinases [ERKs] inhibitor), SB203580 (a p38 mitogen-activated protein kinases [MAPK] inhibitor), SP600125 (a c-Jun N-terminal kinase [JNK] inhibitor) were also used to see the role of MAPKs signaling pathway(s) in IL-1β-induced MMP-9 expression in HEI-OC1 cells. Reverse transcription-polymerase chain reaction and gelatin zymography were used to measure mRNA expression level of MMP-9 and activity of MMP-9, respectively.
Results. Treatment with IL-1β-induced the expression of MMP-9 in a dose- and time-dependent manner. IL-1β (1 ng/mL)-induced MMP-9 expression was inhibited by dexamethasone. Interestingly, p38 MAPK inhibitor, SB203580, significantly inhibited IL-1β-induced MMP-9 mRNA and MMP-9 activity. However, inhibition of JNKs and ERKs had no effect on the IL-1β-induced MMP-9 expression.
Conclusion. These results suggest that the pro-inflammatory cytokine IL-1β strongly induces MMP-9 expression via activation of p38 MAPK signaling pathway in HEI-OC1 cells and the induction was inhibited by dexamethasone.
Keywords. Interleukin-1beta, Matrix metalloproteinase 9, p38 Mitogen-activated protein kinases, Cochlea
Keimyung Author(s)(Kor)
남성일
권택규
Publisher
School of Medicine
Citation
Sung-Il Nam and Taeg-Kyu Kwon. (2014). Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in
Cochlear Cells. Clinical and Experimental Otorhinolaryngology, 7(3), 175–180. doi: 10.3342/ceo.2014.7.3.175
Type
Article
ISSN
1976-8710
DOI
10.3342/ceo.2014.7.3.175
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35462
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
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