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Negative and positive feedback regulation of insulin in glucose-stimulated Ca2+ response in pancreatic beta cells

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Author(s)
Sung Hee-ParkBora LimWon-Ki BaekJae-Hoon BaeDae-Kyu Song
Keimyung Author(s)
Bae, Jae HoonSong, Dae KyuBaek, Won Ki
Department
Dept. of Physiology (생리학)
Dept. of Microbiology (미생물학)
Journal Title
Diabetes Research and Clinical Practice
Issued Date
2007
Volume
77S
Issue
3
Abstract
Secreted insulin from pancreatic beta cells exerts autocrine and paracrine effects within the islets. The present study has
evaluated how exogenous insulin participates in cytosolic Ca2+ response to high glucose, according to glucose concentration at
which insulin is applied. When 100 nM insulin was pretreated to the bath solution containing islet cells in the presence of basal level
of glucose, the elevation of cytosolic Ca2+ concentration ([Ca2+]c) by subsequently applied 10 mM glucose was remarkably
attenuated. In contrast, the glucose-stimulated [Ca2+]c elevation was more potentiated when insulin was superimposed on the high
glucose stimulation. These insulin actions were modestly inhibited by the application of LY294002, the phosphatidylinositol 3-
kinase (PI3-kinase) inhibitor, but not completely, suggesting that another mechanism is also involved. By 100 nM insulin,
phosphorylated form of AMP-activated protein kinases (p-AMPK) was dramatically decreased in basal glucose but increased in
high glucose, when compared with their reciprocal controls. These results may suggest that the extent of AMPK activation may be a
tool for insulin receptors to monitor blood glucose level, with which insulin-induced insulin receptor activation determines the way
to go negatively or positively toward [Ca2+]c.
# 2007 Elsevier Ireland Ltd. All rights reserved.
Keywords: Insulin; Pancreatic beta cell; Insulin receptor; Glucose-stimulated Ca2+ response; AMP-activated protein kinase
1. Introduction
Insulin, which is secreted from pancreatic beta cells,
is an anabolic hormone to act primarily on insulinsensitive
tissues, such as skeletal muscles, liver, and
adipose tissues,
Keimyung Author(s)(Kor)
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Publisher
School of Medicine
Citation
Sung Hee-Park et al. (2007). Negative and positive feedback regulation of insulin in
glucose-stimulated Ca2+ response in pancreatic beta cells. Diabetes Research and Clinical Practice, 77S(3), S143–S149. doi: 10.1016/j.diabres.2007.02.023
Type
Article
ISSN
0168-8227
Source
https://www.sciencedirect.com/science/article/pii/S0168822707002355?via%3Dihub
DOI
10.1016/j.diabres.2007.02.023
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35574
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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