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Enhancing effect of taurine on glucose response in UCP2-overexpressing beta cells

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Author(s)
Lee Sang-HoonLee Hyun-YoungKim So-YeonLee In-KyuSong Dae-Kyu
Keimyung Author(s)
Song, Dae KyuLee, In Kyu
Department
Dept. of Physiology (생리학)
Dept. of Internal Medicine (내과학)
Journal Title
Diabetes Research and Clinical Practice
Issued Date
2004
Volume
66
Issue
Supplement
Abstract
Uncoupling protein 2 (UCP2) is up-regulated in pancreatic beta cells when exposed to long-term high glucose or free fatty
acids, which results in impaired glucose-induced insulin secretion (GIIS). We have evaluated whether taurine pretreatment can
restore impaired GIIS of beta cells overexpressing UCP2 by adenovirus (Ad)-mediated transfection technique. In Ad-Null
control cells, externally applied glucose (10 mM) inhibited ATP-sensitive potassium (KATP) channel activity even in the
presence of 300 mMdiazoxide. In Ad-UCP2 cells, however, glucose failed to inhibit KATP channel activity; despite the response
of KATP channel itself to glibenclamide was normal. The glucose-stimulated increase of cytosolic Ca2+ concentration ([Ca2+]c)
and insulin secretion was also diminished (P < 0.05). When taurine (3 mM) was pretreated for 24 h, the glucose responses of
Ad-UCP2 cells were remarkably restored. The effect of taurine was, however, blocked by CCCP (carbonyl cyanide pchlorophenylhydrazone;
2 mM), a mitochondrial Ca2+ uniporter inhibitor. These results suggest that taurine restores impaired
GIIS in Ad-UCP2 cells, at least partially, by acting on the mechanism for Ca2+ sequestration into the mitochondrial matrix.
# 2004 Elsevier Ireland Ltd. All rights reserved.
Keywords: Uncoupling protein 2; Taurine; Glucose-induced insulin secretion; KATP channel; Ca2+ uniporter
Keimyung Author(s)(Kor)
송대규
이인규
Publisher
School of Medicine
Citation
Lee Sang-Hoon et al. (2004). Enhancing effect of taurine on glucose response in
UCP2-overexpressing beta cells. Diabetes Research and Clinical Practice, 66(Supplement), S69–S74. doi: 10.1016/j.diabres.2003.08.018
Type
Article
ISSN
0168-8227
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S016882270400169X?via%3Dihub
DOI
10.1016/j.diabres.2003.08.018
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35581
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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