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Thiosulfate sulfurtransferase and UDP-glucuronosyltransferase activities in cholestatic rat liver induced by common bile duct ligation

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Author(s)
Jong-Sool IhmYou-Hee Kim
Keimyung Author(s)
Kim, You Hee
Department
Dept. of Biochemistry (생화학)
Journal Title
Experimental and Molecular Medicine.
Issued Date
1997
Volume
29
Issue
4
Abstract
We have investigated the effect of cholestasis on the
hepatic thiosulfate sulfurtransferase (rhodanese) and
UDP-glucuronosyltransferase (UDP-GT) activities in
rats. Rhodanese activities in the liver cytosol, mitochondria
and microsomal fractions as well as in the
rat serum, and UDP-GT activity in the microsome have
been investigated for a period of 42 days after common
bile duct (CBD) ligation. The cytosolic rhodanese
activity showed a significant decrease between the
first through the 42nd day, and the mitochondrial
activity showed a significant decrease between the
7th through the 42nd day after CBD ligation
compared to the activities from the sham operated
control, respectively. In the case of microsomal
preparation, both rhodanese and UDP-GT also
showed significant decrease in their activities after
the ligation for the former enzyme between the 14th
and the 42nd days, and for the latter enzyme
between the third and 42nd days, respectively. On
the other hand, the serum rhodanese activity
increased markedly soon after the ligation, exhibiting
the peak activity after 1 day of CBD ligation with
about 4.6-fold increment. The activity subsequently
decreased gradually reaching to the control level at
the 42nd day post-ligation. Enzyme kinetic
parameters of hepatic rhodanese and UDP-GT were
analyzed using sodium thiosulfate and pnitrophenol
as substrates, respectively, with the
preparations from the 28th day post-ligation. The
results indicated that although the Km values of these
enzymes were about the same as the sham-operated
control, the Vm a x values of the both enzymes decreased
s i g n i f i c a n t l y. These results, therefore, suggest that
the biosynthesis of rhodanese and UDP-GT have
been reduced in response to cholestasis, and that
the elevation of rhodanese activity in the serum is
most likely due to leakage from the liver
subsequent to CBD ligation.
Keywords: thiosulfate sulfurtransferase (rhodanese),
UDP-glucuronosyltransferase, cholestatic rat liver
Keimyung Author(s)(Kor)
김여희
Publisher
School of Medicine
Citation
Jong-Sool Ihm and You-Hee Kim. (1997). Thiosulfate sulfurtransferase and UDP-glucuronosyltransferase
activities in cholestatic rat liver
induced by common bile duct ligation. Experimental and Molecular Medicine., 29(4), 197–201. doi: 10.1038/emm.1997.30
Type
Article
ISSN
1226-3613
DOI
10.1038/emm.1997.30
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35711
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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