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Effects of high taurocholate load on activities of hepatic alcohol metabolizing enzymes

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Author(s)
You Hee KimMi Jeoung Shin
Keimyung Author(s)
Kim, You Hee
Department
Dept. of Biochemistry (생화학)
Journal Title
Experimental and Molecular Medicine.
Issued Date
2002
Volume
34
Issue
2
Abstract
Membrane-associated cytotoxicity induced by hydrophobic
bile salts is a major contributing factor leading
to liver diseases. Administration of ursodeoxycholate
reduces serum liver enzymes in chronic liver diseases
but the nature of this effect is still unclear.
Using alcohol metabolising enzymes as cellular
markers, the hepatotoxic properties of hydrophobic
bile salts and the putative hepatoprotective effect of
ursodeoxycholate was examined. Two animal models
of biliary retention, bile duct obstruction and
choledochocaval fistula was used to investigate the
effect of taurocholate on the hepatic alcohol metabolizing
enzymes: cytosolic alcohol dehydrogenase,
microsomal ethanol oxidizing system, catalase and
aldehyde dehydrogenase before and after the infusion
of taurocholic acid or tauroursodeoxycholic
acid for two days period. Bile duct obstruction was
found to be similar to or slightly exceeds choledochocaval
fistula in the degree of retention. Following
the taurocholic acid infusion, the serum alcohol
dehydrogenase activity as well as microsomal ethanol
oxidizing system and aldehyde dehydrogenase
were greatly increased but the level of cytosolic
alcohol dehydrogenase and catalase activities was
found to be lower in either or both models in comparison
with the control animals. However, the tauroursodeoxycholic
acid infusion did not induce any
significant changes in the levels of all the alcohol
metabolizing enzyme activities in either or both
models. These findings suggest that hydrophobic
taurocholic acid (7a) affects the plasmalemma to
allow leakage of cytosolic alcohol dehydrogenase
into the blood circulation, stimulates the biosynthesis
of microsomal ethanol oxidizing system and
aldehyde dehydrogenase, and suppresses the biosynthesis
of alcohol dehydrogenase and catalase.
But in contrast, the hydrophilic tauroursodeoxycholic
acid (7b) provided hepatoprotective effect.
Keimyung Author(s)(Kor)
김여희
Publisher
School of Medicine
Citation
You Hee Kim and Mi Jeoung Shin. (2002). Effects of high taurocholate load on activities of hepatic alcohol
metabolizing enzymes. Experimental and Molecular Medicine., 34(2), 123–130. doi: 10.1038/emm.2002.18
Type
Article
ISSN
1226-3613
DOI
10.1038/emm.2002.18
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35713
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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