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Interferon regulatory factor-1 is prerequisite to the constitutive expression and IFN-γ-induced upregulation of B7-H1 (CD274)

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Author(s)
Seung-Jin LeeByeong-Churl JangSoo-Woong LeeYoung-Il YangSeong-Il SuhYeong-Min ParkSangtaek OhJae-Gook ShinSheng YaoLieping ChenIn-Hak Choi
Keimyung Author(s)
Jang, Byeong ChurlSuh, Seong Il
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Journal Title
FEBS Lett
Issued Date
2006
Volume
580
Issue
3
Abstract
Majority of cancer cells upregulate co-inhibitory molecule
B7-H1 which confers resistance to anti-tumor immunity,
allowing cancers to escape from host immune surveillance. We
addressed the molecular mechanism underlying the regulation
of cancer-associated B7-H1 expression in response to interferon-
c (IFN-c). Using promoter constructs in luciferase assay,
the region between 202 and 320 bp from the translational start
site is responsible for B7-H1 expression. Electrophoretic mobility
shift assay, site-directed mutagenesis and knockdown experiment
using siRNA revealed that interferon regulatory factor-1
(IRF-1) is primarily responsible for the constitutive B7-H1
expression as well as for the IFN-c-mediated B7-H1 upregulation
in a human lung cancer cell line A549. Additionally,
AG490, a Janus activated kinase/signal transducer and activator
of transcription inhibitor, greatly abolished the responsiveness of
A549 cells to IFN-c by reducing the IRF-1 transcription. Our
findings support a critical role of IRF-1 in the regulation of constitutive
and IFN-c-induced expression of B7-H1 in cancer cells.
2006 Federation of European Biochemical Societies. Published
by Elsevier B.V. All rights reserved.
Keimyung Author(s)(Kor)
장병철
서성일
Publisher
School of Medicine
Citation
Seung-Jin Lee et al. (2006). Interferon regulatory factor-1 is prerequisite to the constitutive
expression and IFN-γ-induced upregulation of B7-H1 (CD274). FEBS Lett, 580(3), 755–762. doi: 10.1016/j.febslet.2005.12.093
Type
Article
ISSN
0014-5793
Source
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2005.12.093
DOI
10.1016/j.febslet.2005.12.093
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35737
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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