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NADPH oxidase is involved in protein kinase CKII down-regulation-mediated senescence through elevation of the level of reactive oxygen species in human colon cancer cells

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Author(s)
Seon Min JeonSung-Jin LeeTaeg Kyu KwonKyung-Jin KimYoung-Seuk Bae
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
FEBS Lett
Issued Date
2010
Volume
584
Issue
14
Abstract
We have shown that protein kinase CKII (CKII) inhibition induces senescence through the p53-
dependent pathway in HCT116 cells. Here we examined the molecular mechanism through which
CKII inhibition activates p53 in HCT116 cells. CKII inhibition by treatment with CKII inhibitor or
CKIIa small-interfering RNA (siRNA) increased intracellular hydrogen peroxide and superoxide
anion levels. These effects were significantly blocked by pretreatment of cells with the antioxidant
N-acetylcysteine. Additionally, NADPH oxidase (NOX) inhibitor apocynin and p22phox siRNA significantly
reduced p53 expression and suppressed the appearance of senescence markers. CKII inhibition
did not affect mitochondrial superoxide generation. These data demonstrate that CKII
inhibition induces superoxide anion generation via NOX activation, and subsequent superoxidedependent
activation of p53 acts as a mediator of senescence in HCT116 cells after down-regulation
of CKII.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Seon Min Jeon et al. (2010). NADPH oxidase is involved in protein kinase CKII down-regulation-mediated
senescence through elevation of the level of reactive oxygen species in human
colon cancer cells. FEBS Lett, 584(14), 3137–3142. doi: 10.1016/j.febslet.2010.05.054
Type
Article
ISSN
0014-5793
Source
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2010.05.054
DOI
10.1016/j.febslet.2010.05.054
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35738
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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