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Pretreatment of indole-3-carbinol augments TRAIL-induced apoptosis in a prostate cancer cell line, LNCaP

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Author(s)
Kye-Im JeonJeong-Keun RihHee Jeong KimYong J. LeeChi-Heum ChoItzhak D. GoldbergEliot M. RosenInsoo Bae
Keimyung Author(s)
Cho, Chi Heum
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Journal Title
FEBS Lett
Issued Date
2003
Volume
544
Issue
1-3
Abstract
Prostate cancer is one of the most common cancers in
men and is the second leading cause of cancer-related deaths in
the USA. Many anti-tumor agents against prostate cancer cells
have been developed, but their unacceptable systemic toxicity to
normal tissues frequently limits their usage in clinics. Several
previous studies have demonstrated that tumor necrosis factorrelated
apoptosis-inducing ligand (TRAIL) can induce cell death
in a variety of transformed cells including prostate cancer cells,
but not normal cells. Indole-3-carbinol (I3C), a phytochemical
that is produced in fruits and vegetables, may play an important
role in the prevention of many types of cancer, including hormone-
related ones such as breast and prostate cancer. In this
study, we examined the potential sensitizing e¡ects of I3C on
TRAIL-mediated apoptosis in a prostate cancer cell line,
LNCaP. When LNCaP cells were incubated with I3C (either
30 or 90 WM) for 24 h and then treated with TRAIL (100 ng/
ml), enhanced TRAIL-mediated apoptosis was observed. The
enhanced apoptosis measured by poly(ADP-ribose) polymerase
and caspase 3 cleavage. We also observed that loss of cell viability
after treatment with I3C/TRAIL is greater compared with
I3C and TRAIL alone. To determine the molecular mechanisms
involved in the enhanced apoptosis, we examined the expression
of two TRAIL death receptors (DR4 and DR5) and two TRAIL
decoy receptors (DcR1 and DcR2). We found that treatment
with I3C induced DR4 and DR5 expression at both transcriptional
and translational levels. These ¢ndings suggest that I3C
may be an e¡ective sensitizer of TRAIL treatment against
TRAIL-resistant prostate cancer cell lines such as LNCaP.
5 2003 Published by Elsevier Science B.V. on behalf of the
Federation of European Biochemical Societies.
Keimyung Author(s)(Kor)
조치흠
Publisher
School of Medicine
Citation
Kye-Im Jeon et al. (2003). Pretreatment of indole-3-carbinol augments TRAIL-induced apoptosis in
a prostate cancer cell line, LNCaP. FEBS Lett, 544(1–3), 246–251. doi: 10.1016/S0014-5793(03)00473-3
Type
Article
ISSN
0014-5793
Source
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/S0014-5793%2803%2900473-3
DOI
10.1016/S0014-5793(03)00473-3
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35739
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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