계명대학교 의학도서관 Repository

Establishment and characterization of cell lines constitutively expressing hepatitis B virus X-protein

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Author(s)
Youngik LeeYongsik BongHaryoung PooYoonik LeeJonggu ParkSuone OhMijin SohnSook LeeUisun ParkNamsoon KimSangwon Hyun
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Gene
Issued Date
1998
Volume
207
Issue
2
Abstract
We prepared human hepatoma cell lines, which expressed the human hepatitis B virus-X gene product. The plasmid pMAMneo-X,
containing an HBV-X gene promoter, an enhancer and a structural gene was constructed.
Transfected HBV-X gene integration and expression were detected by Southern and Northern blotting, as well as by
chloramphenicol acetylase transferase (CAT) assay using various kinds of promoter-CAT reporter systems. HBV-X protein
expression in stable transfectants was confirmed by immunofluorescence microscopy. Transfected cell lines showed permanent
expression of HBV-X proteins. The HBV-X transfectant activated its target promoters in promoter-CAT constructs as reporters.
The HBV-X transfectant enhanced AP-1 transcription factor binding to its target DNA. Therefore, X-transfectants are not only
stable, but also have specific biological functions. Cell cycle analysis by flow cytometry showed that the majority of the transfectant
cells are arrested in the G1 or G2 phase of the cell cycle. These cell lines may be useful in analyzing the biological functions of
HBV-X and its functional role in the formation of hepatocellular carcinomas. © 1998 Elsevier Science B.V.
Keywords: Hepatitis B virus-X protein; Hepatoma cell line; Stable transfectant; DNA-binding activity; Cell cycle analysis;
Hepatocellular carcinoma
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
Youngik Lee et al. (1998). Establishment and characterization of cell lines constitutively
expressing hepatitis B virus X-protein. Gene, 207(2), 111–118. doi: 10.1016/S0378-1119(97)00603-3
Type
Article
ISSN
0378-1119
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0378111997006033?via%3Dihub
DOI
10.1016/S0378-1119(97)00603-3
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35766
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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