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Suppression of glomerulosclerosis by adenovirusmediated IL-10 expression in the kidney

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Author(s)
Y-K ChoiY-J KimH-S ParkK ChoiS-G PaikY-I LeeJ-G Park
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Cancer Gene Therapy
Issued Date
2003
Volume
10
Issue
7
Abstract
Glomerulosclerosis is a common morphologic result seen in
almost all progressed renal diseases, and is the characteristic
change in focal segmental glomerulosclerosis (FSGS).
The most convincing hypothesis for glomerulosclerosis is
cytokine-mediated injury by infiltrating immune cells in the
glomerulus and tubulointerstitial area. This study investigated
whether the anti-inflammatory effect of interleukin-10
(IL-10) when expressed by a recombinant adenoviral vector
can prevent the onset of glomerulosclerosis in FGS/Kist mice
(an animal model with naturally occurring renal failure
initiated by FSGS). Each group of mice received recombinant
adenoviruses encoding human IL-10 (Ad:hIL-10) by
intraparenchymal injection at 6 weeks and were examined for
cytokine expression, glomerular sclerotic index, and proteinuria.
After injection of Ad:hIL-10 to the kidney, IL-10
expression was found to last over 20 days. Mice treated
with Ad:hIL-10 were shown to have a significant reduction in
the glomerular sclerotic index at 10 weeks when compared to
control groups. The level of proteinuria in Ad:hIL-10-treated
mice was also significantly reduced. About 50% of the urine
samples of naive and Ad:LacZ-treated groups had severe
levels of proteinuria. By contrast, at 10 weeks the group
treated with Ad:hIL-10 had lower levels of proteinuria and
transforming growth factor-b1 (TGF-b1) expression. These
results demonstrate that IL-10 effectively prevents the
development of glomerulosclerosis in FGS/Kist mice, and
IL-10 gene therapy may be of use for the treatment of renal
failure.
Gene Therapy (2003) 10, 559–568. doi:10.1038/sj.gt.3301926
Keywords: focal segmental glomerulosclerosis; interleukin-10; adenovirus
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
Y-K Choi et al. (2003). Suppression of glomerulosclerosis by adenovirusmediated
IL-10 expression in the kidney. Cancer Gene Therapy, 10(7), 559–568. doi: 10.1038/sj.gt.3301926
Type
Article
ISSN
0969-7128
Source
http://www.nature.com/articles/3301926
DOI
10.1038/sj.gt.3301926
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35773
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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