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Pathological Dynamics of Activated Microglia Following Medial Forebrain Bundle Transection

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Author(s)
BYUNG P. CHODAE Y. SONGSHUEI SUGAMADONG H. SHINYOSHINORI SHIMIZUSUNG S. KIMYOON S. KIMTONG H. JOH
Keimyung Author(s)
Shin, Dong Hoon
Department
Dept. of Preventive Medicine (예방의학)
Brain Research Institute (뇌연구소)
Journal Title
Glia
Issued Date
2006
Volume
53
Issue
14
Abstract
KEY WORDS
axotomy; phagocytosis; ED1; MHC II; migration; dopami-
nergic neuron; Parkinson’s disease

To elucidate the role and pathological dynamics of activated
microglia, this study assessed the phagocytic, immunopheno-
typic, morphological, and migratory properties of activated
microglia in the medial forebrain bundle (MFB) axotomized
rat brain. Activated microglia were identified using two dif-
ferent monoclonal antibodies: ED1 for phagocytic activity
and OX6 for major histocompatibility complex (MHC) class
II. Phagocytic microglia, characterized by ED1-immuno-
reactivity or ED1- and OX6-immunoreactivity, appeared in
the MFB and substantia nigra (SN) as early as 1–3 days
post-lesion (dpl), when there was no apparent loss of SN
dopamine (DA) neurons. Thereafter, a great number of acti-
vated microglia selectively adhered to degenerating axons,
dendrites and DA neuronal somas of the SN. This was fol-
lowed by significant loss of these fibers and nigral DA neu-
rons. Activation of microglia into phagocytic stage was most
pronounced between 1428 dpl and gradually subsided, but
phagocytic microglia persisted until 70 dpl, the last time
point examined. ED1 expression preceded MHC II expres-
sion in phagocytic microglia. All phagocytic microglia stick-
ing to DA neurons showed activated but ramified form with
enlarged somas and thickened processes. They were re-
cruited to the SNc from cranial, dorsal and ventral aspects
along various structures and finally stuck to DA neurons of
the SNc. Characteristic rod-shaped microglia in the white
matter were thought to migrate a long distance. The present
study strongly suggests that neurons undergoing delayed
neurodegeneration may be phagocytosed by numerous phago-
cytic, ramified microglia at various sites where specific sur-
face signals are exposed or diffusible molecules are
released.
V
V
C
2005 Wiley-Liss, Inc
Keimyung Author(s)(Kor)
신동훈
Publisher
School of Medicine
Citation
BYUNG P. CHO et al. (2006). Pathological Dynamics of Activated Microglia Following
Medial Forebrain Bundle Transection. Glia, 53(14), 92–102. doi: 10.1002/glia.20265
Type
Article
ISSN
0894-1491
Source
http://dx.doi.org.proxy.dsmc.or.kr/10.1002/glia.20265
DOI
10.1002/glia.20265
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35780
Appears in Collections:
3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
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